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Identification of microsatellite markers in the rye genome


The rye genomic library, which consists of DNA fragments in the range of 0.5–1.1 kb, was screened for the presence of tri-and tetranucleotide and compound microsatellites. Of the 1,600,000 clones analysed, 102 clones were positive and 41 were suitable for SSR primer pair design. Twenty-six primer pairs amplified specific products, and six of them were capable of detecting polymorphism among 30 rye accessions of different genetic backgrounds. Using a set of Chinese Spring-Imperial wheat-rye addition lines, it was possible to locate 3 newly identified microsatellites on chromosomes 3R, 4R and 7R.



simple sequence repeat


inter-simple sequence repeat


selective amplification of microsatellite polymorphic loci


expressed sequence tags


insert size [bp]


genome size [bp]


genetic similarity


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Correspondence to Monika Rakoczy-Trojanowska.

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Bolibok, H., Rakoczy-Trojanowska, M., Wyrzykowska, M. et al. Identification of microsatellite markers in the rye genome. Cell. Mol. Biol. Lett. 11, 291–298 (2006).

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Key words

  • Rye
  • Genomic library
  • SSR
  • Microsatellite
  • Wheat-rye addition lines