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Identification of microsatellite markers in the rye genome

Cellular & Molecular Biology Letters volume 11, pages291–298 (2006) | Download Citation

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Abstract

The rye genomic library, which consists of DNA fragments in the range of 0.5–1.1 kb, was screened for the presence of tri-and tetranucleotide and compound microsatellites. Of the 1,600,000 clones analysed, 102 clones were positive and 41 were suitable for SSR primer pair design. Twenty-six primer pairs amplified specific products, and six of them were capable of detecting polymorphism among 30 rye accessions of different genetic backgrounds. Using a set of Chinese Spring-Imperial wheat-rye addition lines, it was possible to locate 3 newly identified microsatellites on chromosomes 3R, 4R and 7R.

Abbreviations

SSR:

simple sequence repeat

ISSR:

inter-simple sequence repeat

SAMPL:

selective amplification of microsatellite polymorphic loci

EST:

expressed sequence tags

IS:

insert size [bp]

GmS:

genome size [bp]

GS:

genetic similarity

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Affiliations

  1. Department of Plant Genetics, Breeding and Biotechnology, Warsaw Agricultural University, Nowoursynowska 166, 02-787, Warszawa, Poland

    • Hanna Bolibok
    • , Monika Rakoczy-Trojanowska
    •  & Małgorzata Wyrzykowska

  2. Plant Breeding and Acclimatization Institute, Radzików, 05-870, Błonie, Poland

    • Magdalena Radecka
    •  & Wacław Orczyk

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Corresponding author

Correspondence to Monika Rakoczy-Trojanowska.

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Key words

  • Rye
  • Genomic library
  • SSR
  • Microsatellite
  • Wheat-rye addition lines

Cellular & Molecular Biology Letters

ISSN: 1689-1392

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