Skip to main content
Download PDF

Studies on genetic changes in rye samples (Secale cereale L.) maintained in a seed bank

Cellular & Molecular Biology Letters volume 11, pages 338–347 (2006)Cite this article

Abstract

The aim of this study was to identify genetic changes in rye seeds induced by natural ageing during long-term storage and consecutive regeneration cycles under gene bank conditions. Genomic DNA from four rye samples varying in their initial viability after one and three cycles of reproduction was analyzed by AFLP (amplified fragment length polymorphism) fingerprinting. Seven EcoRI/MseI primer combinations defined 663 fragments, and seven PstI/MseI primer combinations defined 551 fragments. The variation in the frequency of the seventy-four EcoRI/MseI bands was statistically significant between samples. These changes could be attributed to genetic changes occurring during storage and regeneration. However, the PstI/MseI fragments appeared to be uninfluenced by seed ageing, regeneration and propagation. A combined Principle Coordinate Analysis revealed differences between samples with different initial viability. We showed that materials with low initial viability differ in their response from highly viable ones, and that the changes exhibited in the former case are preserved through regeneration cycles.

Abbreviations

AFLP:

amplified fragment length polymorphism

DZ:

Dańkowskie Złote

PCA:

principal component analysis

RAPD:

random amplified polymorphic DNA

STS:

sequence-tagged sites

References

  1. Shoen, D.J., Jacques, L.D. and Bataillon, T.M. Deleterious mutation accumulation and regeneration of genetic resources. Proc. Natl. Acad. Sci. USA 95 (1998) 394–399.

    Article  Google Scholar 

  2. Roos, E.E. Genetic shifts in mixed bean populations. I. Storage effects. Crop Sci. 24 (1984a) 240–244.

    Article  Google Scholar 

  3. Roos, E.E. Genetic shifts in mixed bean populations. II. Effects of regeneration. Crop Sci. 24 (1984b) 711–715.

    Article  Google Scholar 

  4. Roos, E.E. and Rincker, C.M. Genetic stability in ‘Pennlate’ orchardgrass seed following artificial ageing. Crop Sci. 22 (1988) 611–613.

    Article  Google Scholar 

  5. Stoyanova, S.D. Genetic shift and variations of gliadins induced by seed ageing. Seed Sci. Technol. 19 (1991) 363–371.

    Google Scholar 

  6. Stoyanova, S.D. Effects of seed ageing and regeneration on the genetic composition of wheat. Seed Sci. Technol. 20 (1992) 489–496.

    Google Scholar 

  7. Stoyanova, S.D. Variation of gliadins in wheat cultivars associated with seed survival and multiplication. Seed Sci. Technol. 24 (1996) 115–126.

    Google Scholar 

  8. Bednarek, P.T., Chwedorzewska, K.J. and Puchalski, J. Preliminary molecular studies on genetic changes in rye seeds due to long-term storage and regeneration. In: Challenges in Rye Germplasm Conservation Ed. by. T. Gass, W. Podyma, J. Puchalski, S.A. Eberhart, International Plant Genetic Resources Institute, Rome: (1998) 54–61.

    Google Scholar 

  9. Shatters, R.G. jr., Schweder, M.E., West, S.H., Abdelghany, A. and Smith, R.L. Environmentally induced polymorphisms detected by RAPD analysis of soybean seed DNA. Seed Science Research Seed Sci. Technol. 5 (1995) 106–116.

    Google Scholar 

  10. Chwedorzewska, K.J., Bednarek, P.T., Puchalski, J. and Krajewski, P. AFLP profiling of long-term stored and regenerated rye genebank samples. Cell. Mol. Biol. Lett. 7A (2002) 457–463.

    Google Scholar 

  11. Chwedorzewska, K.J., Bednarek, P.T. and Puchalski, J. Studies on specific rye genome regions due to seed ageing and regeneration. Cell. Mol. Biol. Lett. 7A (2002) 569–576.

    Google Scholar 

  12. Chebotar, S., Röder, M.S., Korzun, V. and Börner, A. Genetic integrity of ex situ genebank collections. Cell. Mol. Biol. Lett. 7A (2002) 437–444.

    Google Scholar 

  13. Börner, A., Chebotar, S. and Korzun, V. Molecular characterization of the genetic integrity of wheat (Triticum aestivum L.) germplasm after long-term maintenance. Theor. Appl. Genet. 100 (2000) 494–497.

    Article  Google Scholar 

  14. Castiglioni, P., Ajmone-Marsan, P., van Wijk, R. and Motto, M. AFLP markers in a molecular linkage map of maize: codominant scoring and linkage group ditstribution. Theor. Appl. Genet. 99 (1999) 425–431.

    CAS  Article  Google Scholar 

  15. Young, W.P., Schupp, J.M. and Keim, P. DNA methylation and AFLP marker distribution in the soybean genome. Theor. Appl. Genet. 99 (1999) 785–790.

    CAS  Article  Google Scholar 

  16. Vos, P., Hogers, R., Bleeker, M., van de Lee, T., Hormes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M. and Zabeau, M. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 21 (1995) 4407–4414.

    Google Scholar 

  17. Bednarek, P.T., Kubicka, H. and Zawada, M. Morphological, cytological and BSA-based testing on limited segregation population AFLPs. Cell. Mol. Biol. Lett. 7B (2002) 635–648.

    Google Scholar 

  18. Bednarek, P.T., Lewandowska, R., Kubicka, H. and Masojć, P. Linkage groups and the indirect chromosome location of cms-P-linked AFLPs. Cell. Mol. Biol. Lett. 7B (2002) 721–736.

    Google Scholar 

  19. Mardia, K.V., Kent, J.T. and Bibby, J.M. Multivariate analysis. Academic Press, London. (1979).

    Google Scholar 

  20. Bednarek, P.T., Lewandowska, R., Gołas, T. and Paśnik, M. The chromosomal location of rye AFLP bands. Cell. Mol. Biol. Lett. 8 (2003) 955–962.

    PubMed  CAS  Google Scholar 

  21. Vuylsteke, M., Mank, R., Antonise, R., Bastiaans, R., Senior, M.L., Stuber, C.W., Melchinger, A.E., Lubberstedt, T., Xia, X.C., Stam, P., Zabeau, M. and Kuiper, M. Two high-density AFLP linkage maps of Zea mays L.: analysis of distribution of AFLP markers. Theor. Appl. Genet. 99 (1999) 921–935.

    CAS  Article  Google Scholar 

  22. Li, Y-CH., Korol, A.B., Fahima, T., Beiles, A. and Nevo, E. Microsatellites: genomic distribution, putative functions and mutational mechanisms: a review. Mol. Ecol. 11 (2002) 2453–2465.

    PubMed  CAS  Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Botanical Garden — Centre for Biological Diversity Conservation of the Polish Academy of Sciences, Prawdziwka 2, 02-973, Warsaw, Poland

    Katarzyna J. Chwedorzewska, Piotr T. Bednarek, Renata Lewandowska & Jerzy Puchalski

  2. Institute of Plant Genetics, Polish Academy of Science, Strzeszyńska 34, Poznań, Poland

    Paweł Krajewski

Authors
  1. Katarzyna J. Chwedorzewska
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Piotr T. Bednarek
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Renata Lewandowska
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Paweł Krajewski
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jerzy Puchalski
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Katarzyna J. Chwedorzewska.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Chwedorzewska, K.J., Bednarek, P.T., Lewandowska, R. et al. Studies on genetic changes in rye samples (Secale cereale L.) maintained in a seed bank. Cell Mol Biol Lett 11, 338–347 (2006). https://doi.org/10.2478/s11658-006-0028-0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.2478/s11658-006-0028-0

Key words

  • Rye
  • Secale cereale L
  • Seed storage and propagation
  • AFLP

Cellular & Molecular Biology Letters

ISSN: 1689-1392

Contact us