Romanos, M.A. Advances in the use of Pichia pastoris for high level gene expression. Curr. Opin. Biotechnol.
6 (1995) 527–533.
Article
CAS
Google Scholar
Escamilla-Treviño, L.L., Viader-Salvadó, J.M. and Guerrero-Olazarán M. Producción de proteínas recombinantes en Pichia pastoris. CIENCIA UANL
2 (1999) 27–33.
Google Scholar
Cregg, J.M., Barringer, K.J., Hessler, A.Y. and Madden, K.R. Pichia pastoris as a host for transformations. Mol. Cell. Biol.
5 (1985) 3376–3385.
PubMed
CAS
Google Scholar
Clare, J.J., Rayment, F.B., Ballantine, S.P., Sreekrishna, K. and Romanos M.A. High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the gene. Biotechnology
9 (1991) 455–60.
Article
PubMed
CAS
Google Scholar
Clare, J.J., Sreekrishna, K. and Romanos M. Expression of tetanus toxin fragment C. Methods Mol. Biol.
103 (1998) 193–208.
PubMed
CAS
Google Scholar
Brankamp, R.G., Sreekrishna, K., Smith, P.L., Blankenship, D.T. and Cardin, A.D. Expression of a synthetic gene encoding the anticoagulant-antimetastatic proteinghilanten by the methylotropic yeast Pichia pastoris. Protein Expr. Purif.
6 (1995) 813–820.
Article
PubMed
CAS
Google Scholar
Romanos, M., Scorer, C., Sreekrishna, K. and Clare, J. The generation of multicopy recombinant strains. in: Pichia Protocols. Methods in Molecular Biology. (Higgins, D.R. and Cregg, J.M., Eds.) Vol. 103, Humana Press Inc, Totowa, NJ, 1998, 55–72.
Chapter
Google Scholar
Sreekrishna, K., Brankamp, R.G., Kropp, K.E., Blankenship, D.L., Tsay, J.-T., Smith, P.L., Wierschke, J.D., Subramaniam, A. and Birkenberger, L.A. Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris. Gene
190 (1997) 55–62.
Article
PubMed
CAS
Google Scholar
Scorer, C.A., Buckholz, R.G., Clare, J.J. and Romanos, M.A. The intracellular production and secretion of HIV-1 envelope protein in the methylotrophic yeast Pichia pastoris. Gene
136 (1993) 111–119.
Article
PubMed
CAS
Google Scholar
Scorer, C.A., Clare J.J., Macombie, R., Romanos, M.A. and Sreekrishna, K. Rapid selection using G418 of high copy number transformats of Pichia pastoris for high-level foreign gene expression. Biotechnology
12 (1994) 181–184.
Article
PubMed
CAS
Google Scholar
Vos, P., Hogers, R., Bleeker, M., Reijans, V., Van de Lee, T., Hornes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M. and Zabeau, M. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res.
23 (1995) 4407–4414.
PubMed
CAS
Google Scholar
Lin, J.J. and Kuo, J. AFLP: a novel PCR-based assay for plant and bacterial DNA fingerprinting. Focus
17 (1995) 66–70.
Google Scholar
Becker, J., Vos, P., Kuiper, M., Salamini, F. and Heun, V. Combined mapping of AFLP and RFLP markers in barley. Mol. Gen. Genet.
249 (1995) 65–73.
Article
PubMed
CAS
Google Scholar
Janssen, P., Coopman, R., Huys, G., Swings, J., Bleeker, M., Vos, P., Zabeau, M. and Kersters, K. Evaluation of the DNA fingerprinting method AFLP as a new tool in bacterial taxonomy. Microbiol.
142 (1996) 1881–1893.
Article
CAS
Google Scholar
Ganter, P.F. and de Barros-Lopes, M. The use of anonymous DNA markers in assessing worldwide relatedness in the yeast species Pichia kluyveri Bedford and Kudrjavzev. Can. J. Microbiol.
46 (2000) 967–980.
Article
PubMed
CAS
Google Scholar
Anonymous. Pichia expression kit: a manual of methods for expression of recombinant proteins in Pichia pastoris. Invitrogen. Available from: URL: http://www.invitrogen.com/content/sfs/manuals/pich_man.pdf
Cregg, J.M. and Russell, K.A. Transformation. in: Pichia Protocols. Methods in Molecular Biology. (Higgins, D.R. and Cregg, J.M., Eds.) Vol. 103, Humana Press Inc, Totowa, NJ, 1998, 27–39.
Chapter
Google Scholar
Escamilla-Treviño, L.L., Viader-Salvadó J.M., Barrera-Saldaña H.A. and Guerrero-Olazarán, M. Biosynthesis and secretion of recombinant human growth hormone in Pichia pastoris. Biotechnol. Lett.
22 (2000) 109–114.
Article
Google Scholar
Hecker, K.H. and Roux, K.H. High and low annealing temperatures increase both specificity and yield in touchdown and stepdown PCR. BioTechniques
20 (1996) 478–485
PubMed
CAS
Google Scholar
Felsenstein, J. PHYLIP Phylogeny Inference Package (Version 3.2). Cladistics
5 (1989) 164–166.
Google Scholar
Sneath, P.H.A. and Sokal, R.R. Numerical Taxonomy: the principles and practice of numerical classification. W.H. Freeman and Company, San Francisco, 1973, 230–234.
Google Scholar
Sreekrishna, K. Strategies for optimizing protein expression and secretion in the methylotrophic yeast Pichia pastoris. in: Industrial microorganisms: basic and applied molecular genetics. (Balz, R.H., Hegeman, G.D. and Skatrud, P.L., Eds.) American Society for Microbiology, Washington, D.C., 1993, 119–126.
Google Scholar
Knorr, C., Cheng, H.H., and Dodgson, J.B. DNA cloning and sequence analysis of chicken AFLP. Anim. Genet.
32 (2001) 156–159.
Article
PubMed
CAS
Google Scholar