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A comparison of the in vitro antimicrobial activity of liposomes containing meropenem and gentamicin

Cellular & Molecular Biology Lettersvolume 11, Article number: 360 () | Download Citation

Abstract

The antimicrobial activity of eight cationic, two neutral and three anionic liposome compositions containing meropenem and gentamicin was tested in vitro in broth and serum medium. The cationic formulations showed better antibacterial efficacy against both Gram-positive and Gram-negative bacteria than the anionic and neutral ones, regardless of the encapsulated drug. The most effective formulations were the cationic PC/DOPE/DOTAP 3:4:3 and PC/Chol/DOTAP 3:4:3, as the MICs with meropenem were 2 to 4 times lower than those of the free drug.

Abbreviations

Chol:

cholesterol

DDAB:

dimethyldioctadecylammoniumbromide salt

DMPG:

1,2-dimyristoyl-sn-glycero-3-phosphoglycerol

DOPE:

1,2-dioleoyl-sn-glycero-3-phosphoethanolamine

DOTAP:

1,2-dioleoyloxy-3-trimethyl ammonium-propane

DPPC:

1,2-diplamitoyl-sn-glycero-3-phosphocholine

GE:

gentamicin

MEM:

meropenem

MHB:

Mueller Hinton II Broth

MLVs:

the multilamellar vesicles

MIC:

minimum inhibitory concentration

MPS:

the mononuclear phagocytic system

NBS:

normal bovine serum

NCCLS:

National Committee for Clinical Laboratory Standards

OPA:

o-phthaldialdehyde

PBS:

phosphate-buffered solution

PC:

phosphatidylcholine

PI:

phosphatidyl inositiol

SA:

octadecylamine

ULVs:

unilamellar vesicles

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Affiliations

  1. Institute of Genetics and Microbiology, University of Wrocław, Przybyszewskiego 63/77, 51-148, Wrocław, Poland

    • Zuzanna Drulis-Kawa
    • , Agata Dorotkiewicz-Jach
    •  & Włodzimierz Doroszkiewicz

  2. Institute of Biochemistry and Molecular Biology, University of Wrocław, 51-148, Wrocław, Poland

    • Jerzy Gubernator
    •  & Arkadiusz Kozubek

  3. Lower Silesian Centre of Paediatrics in Wrocław, 51-147, Wrocław, Poland

    • Zuzanna Drulis-Kawa

  4. Academic Centre for Biotechnology of Supramolecular Lipid Aggregates, 51-148, Wrocław, Poland

    • Jerzy Gubernator

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Correspondence to Zuzanna Drulis-Kawa.

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Key words

  • Liposomes
  • Meropenem
  • Gentamicin
  • Bacteria

Cellular & Molecular Biology Letters

ISSN: 1689-1392

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