- Short Communication
The effect of auxins (IAA and 4-Cl-IAA) on the redox activity and medium pH of Zea mays L. root segments
Cellular & Molecular Biology Lettersvolume 11, pages376–383 (2006)
Indole-3-acetic acid (IAA) and 4-chloroindole-3-acetic acid (4-Cl-IAA) were tested at different concentrations and times for their capacity to change the redox activity and medium pH of maize root segments. The dose-response surfaces (dose-response curves as a function of time) plotted for redox activity and changes in medium pH (expressed as ΔpH) had a similar shape for both auxins, but differed significantly at the optimal concentrations. With 4-Cl-IAA, the maximal values of redox activity and medium pH changes were observed at 10−10 M, which was a 100-fold lower concentration than with IAA. Correlations were observed between redox activity and medium pH changes at the optimal concentrations of both IAA and 4-Cl-IAA. The results are discussed herein, taking into account both the concentration of the auxins and the effects produced by them.
- HCF (III):
Crane, F.L., Goldenberg, H. and Morré, D.J. Dehydrogenases of the plasma membrane. Subcell. Biochem. 6 (1979) 345–399.
Lüthje S., Döring, O., Heuer, S., Lüthen, H. and Böttger, M. Oxidoreductases in plant plasma membranes. Biochim. Biophys. Acta 1331 (1997) 81–102.
Medina, M.A., Del Castillo-Olivares, A. and NúÑez De Castro, I. Multifunctional plasma membrane redox systems. BioEssays 19 (1997) 977–984.
Bérczi, A. and Møller, I.M. Redox enzymes in the plant plasma membrane and their possible roles. Plant Cell Env. 23 (2000) 1287–1302.
Craig, T.A. and Crane, F.L. Evidence for transplasma membrane electron transport system in plant cells. Proc. Ind. Acad. Sci. 90 (1981) 150–155.
Federico, R. and Giartosio, C.E. A transplasmamembrane electron transport system in maize roots. Plant Physiol. 73 (1983) 182–184.
Rubinstein, B., Stern, A.I. and Stout, R.G. Redox activity at the surface of oat root cells. Plant Physiol. 76 (1984) 386–391.
Menckhoff, M. and Lüthje, S. Transmembrane electron transport in sealed and NAD(P)H-loaded right-side-out plasma membrane vesicles isolated from maize (Zea mays L.) roots. J. Exp. Bot. 55 (2004) 1343–1349.
Rubinstein, B. and Stern, A.I. Relationship of transplasmamembrane redox activity to proton and solute transport by roots of Zea mays. Plant Physiol. 80 (1986) 805–811.
Böttger, M. and Lüthen, H. Possible linkage between NADH-oxidation and proton secretion in Zea mays L. roots. J. Exp. Bot. 37 (1986) 666–675.
Barr, R. The possible role of redox-associated protons in growth of plant cells. J. Bioenerg. Biomemb. 23 (1991) 443–467.
Döring, O., Lüthje, S., Hilgendorf, F. and Böttger, M. Membrane depolarization by hexacyanoferrate (III), hexabromoiridiate (IV) and hexachloroiridiate (IV). J. Exp. Bot. 41 (1990) 1055–1061.
Grabov, A. and Böttger, M. Are redox reactions involved in regulation of K+ channels in the plasma membrane of Limnobium stoloniferum root hairs? Plant Physiol. 105 (1994) 927–935.
Barr, R. and Böttger, M. The effect of chloro-derivatives of indoleactic acid on plasma membrane electron transport and proton excretion. Proc. Ind. Acad. Sci. 99 (1991) 129–136.
Carrasco-Luna, J., Calatayud, A., González-Darós, F. and del Valle-Tascón, S. Hexacyanoferrate (III) stimulation of elongation in coleoptile segments from Zea mays L. Protoplasma. 184 (1995) 63–71
Davies, P.J. Plant hormones. Biosynthesis, signal transduction, action. eds. Kluwer Academic Publishers, (2004) 204–220.
Taiz, L. and Zeiger, E. Plant Physiology. 3rd edn. Sinauer Associates, Inc., Publishers (2002).
Engvild, K.C. Natural chlorinated auxins labelled with radioactive chloride in immature seeds. Physiol. Plant. 34 (1975) 286–287.
Engvild, K.C. Simple identification of natural chlorinated auxin in pea by thin layer chromatography. Physiol. Plant. 48 (1980) 435–437.
Engvild, K.C., Egsgaard, H. and Larsen, E. Gass chromatographic-mass spectrometric identification of 4-chloroindole-3-acetic acid methyl ester in immature green peas. Physiol. Plant. 42 (1978) 365–368.
Engvild, K.C., Egsgaard, H. and Larsen, E. Determination of 4-chloroindole-3-acetic acid methyl ester in Lathyrus, Vicia and Pisum by gas chromatography-mass spectrometry. Physiol. Plant. 48 (1980) 499–503.
Hofinger, M. and Böttger, M. Identification by GC-MS of 4-chloroindolilacetic acid and its methyl ester in immature Vicia faba seeds. Phytochem. 18 (1979) 653–654.
Katayama, M., Thiruvikraman, S.V. and Marumo, S. Identification of 4-chloroindole-3-acetic acid and its methyl ester in immature seeds of Vicia amurensis (the tribe Viciaeae) and their absence from three species of Phaseoleae. Plant Cell Physiol. 28 (1987) 383–386.
Böttger, M., Engvild, K.C. and Soll, H. Growth of Avena coleoptiles and pH drop of protoplast suspensions induced by chlorinated indoleacetic acids. Planta 140 (1978) 89–92.
Pless, T., Böttger, M., Hedden, P. and Grabe, J. Occurrence of 4-Cl-indoleacetic acid in broad beans and correlation of its levels with seeds development. Plant Physiol. 74 (1984) 320–323.
Ahmad, A., Anderson, A.S. and Engvild, K.C. Rooting, growth and ethylene evolution of pea cuttings in response to chloroindole auxins. Physiol. Plant. 69 (1987) 137–140.
Hatano, T., Katayama, M. and Marumo, S. 5,6-dichloroindole-3-acetic acid as a potent auxin: its synthesis and biological activity. Experientia 43 (1987) 1237–1239.
Fischer, C., Lüthen, H., Böttger, M. and Hertel, R. Initial transient growth inhibition in maize coleoptiles following auxin application. J. Plant Physiol. 141 (1992) 88–92.
Rescher, U., Walther, A., Schiebl, C. and Klämbt, D. In vitro binding affinities of 4-chloro-, 2-methyl-, 4-methyl-, and 4-ethyl-indoleacetic acid to auxin-binding protein 1 (ABP1) correlate with their growth-stimulating activities. J. Plant Growth Reg. 15 (1996) 1–3.
Karcz, W., Lüthen, H. and Böttger, M. Comparative investigation of IAA and 4-Cl-IAA-induced growth and proton secretion in maize coleoptile segments. Plant Physiol. and Biochem. Spec. Iss. Abstract S01-14, (1996) 7
Karcz, W., Lüthen, H. and Böttger, M. Effect of IAA and 4-Cl-IAA on growth rate in maize coleoptile segments. Acta Physiol. Plant. 21 (1999) 133–139.
Karcz, W. and Burdach, Z. A comparision of the effects of IAA and 4-Cl-IAA on growth, proton secretion and membrane potential in maize coleoptile segments. J. Exp. Bot. 53 (2002) 1089–1098.
Böttger, M. and Hilgendorf, F. Hormone action on transmembrane electron and H+ transport. Plant Physiol. 86 (1988) 1038–1043
Lüthen, H., Hilgendorf, F. and Böttger, M. Effect of auxin on growth, proton secretion and transmembrane electron transfer in intact maize roots. in: Structural and functional aspects of transport in roots (Loughman B.C. et al., Eds.), Kluwer Academic Publishers, 1989, 63–67.
Hilgendorf, F. and Böttger, M. Influence of temperature on proton secrection and hexacyanoferrate (III) reduction of Zea mays L. roots. Plant Physiol. 101 (1993) 1340–1353.
Lüthen, H. and Böttger, M. Induction of elongation in maize coleoptiles by hexachloroiridate and its interrelation with auxin and fusicoccin action. Physiol. Plant. 89 (1993) 77–86.