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Schistosoma japonicum: The design and experimental evaluation of a multivalent DNA vaccine
Cellular & Molecular Biology Letters volume 11, pages 449–460 (2006)
Abstract
The aim of this study was to construct and evaluate the immunity efficacy of the DNA multivalent vaccine pVIVO2SjFABP-23. The vaccine was constructed and produced as follows. Forty BALB/c mice were divided into four groups designated pVIVO2, pVIVO2Sj23, pVIVO2SjFABP and pVIVO2SjFABP-23. Each mouse was immunized with 100 μg of the corresponding plasmid DNA by intramuscular injection. 28 days post-vaccination, the mice were challenged with S. japonicum cercariae, and the worm and egg burdens were determined 42 days post-challenge. Serum samples were collected from all the mice before and after vaccination and at the end of the experiment, and used for antibody detection. The IFN-γ and IL-4 levels were quantified in the supernatants of specifically stimulated spleen cells. The number of worms was reduced by 52%, 40% and 42% in mice respectively immunized with pVIVO2SjFABP-23, pVIVO2Sj23 or pVIVO2SjFABP. A respective 61%, 38% and 39% egg reduction was determined relative to those mice that only received the empty pVIVO2 plasmid. pVIVO2SjFABP-23 immunization increased IgG levels against SWAP and SEA. Increased IFN-γ levels were detected in the supernatant of specific stimulated spleen cells from mice immunized with the 3 different constructs. The multivalent DNA vaccine developed induced higher levels of protection than the two monovalent tested vaccines.
Abbreviations
- FABP:
-
fatty acid binding protein
- SEA:
-
soluble egg antigen
- Sj:
-
Schistosoma japonicum
- SOE:
-
splicing by overlapping extension
- SWAP:
-
soluble adult worm antigens
- WHO:
-
world health organization
- 23:
-
23 kDa membrane protein
References
Ross, A.G. Schistosomiasis. N. Engl. J. Med. 346 (2002) 1212–1220.
Chitsulo, L., Engels, D., Montresor, A. and Savioli, L. The global status of schistosomiasis and its control. Acta Trop. 77 (2000) 41–51.
WHO Expert Committee. Prevention and control of schistosomiasis and soil-transmitted helminthiasis: report of the WHO expert committee. WHO Technical Report Series No. 912. (World Health Organization, Geneva, Switzerland), 2002, 1–57.
Van der Werf, M.J. and De Vlas, S.J. Quantification of clinical morbidity associated with Schistosoma infection in sub-Saharan Africa. Acta Trop. 86 (2003) 125–139.
Tan, H.Z., Yang, M.X., Wu, Z.G., Zhou, J., Liu, A.Z., Li, S.Q., Yang, T.B., Zhou, Y.B., and Sun, Z.Q. Rapid screening method for Schistosoma japonicum infection using questionnaires in flood area of the People’s Republic of China. Acta Trop. 90 (2004) 1–9.
Gurunathan, S., Klinman, D. and Seder, R. DNA vaccines immunology, application and optimization. Annu Rev. Immunol. 18 (2000) 927–974.
Alarcon, J., Waine, G. and McManus, D.P. DNA vaccines: technology and application as anti-parasite and antimicrobial agents. Adv. Parasitol. 42 (1999) 343–410.
Kofta, W. and Wedrychowicz, H. c-DNA vaccination against parasitic infections. advantages and disadvantages. Vet. Parasitol. 100 (2001) 3–12.
Rosas, G., Fragoso, G., Garate, T., Hernandez, B., Ferrero, P., Foster-Cuevas, M., Parkhouse, R.M., Harrison, L.J., Briones, S.L., Gonzalez, L.M. and Sciutto, E. Protective immunity against Taenia crassiceps murine cysticercosis induced by DNA vaccination with a Taenia saginata tegument antigen. Microbes Infect. 4 (2002) 1417–1426.
Wang, Q.M., Sun, S.H., Hu, Z.L., Wu, D. and Wang, Z.C. Immune response and protection elicited by DNA immunization against Taenia cysticercosis. Vaccine. 21 (2003) 1672–1680.
Waine, G., Alarcon, J. and McManus, D.P. Genetic immunization of mice with DNA encoding the 23 kDa transmembrane surface protein of Schistosoma japonicum (Sj23) induces antigen-specific immunoglobulin G antibodies. Parasite Immunol. 21 (1999) 377–381.
Siddiqui, A.A., Phillips, T., Charest, H., Podesta, R.B., Quinlin, M.L., Pinkston, J.K., Lloyd, J.D., Pompa, J., Villalovos, R.M. and Paz, M. Enhancement of Sm-p80 (large subunit of calpain) induced protective immunity against Schistosoma mansoni through co-delivery of interleukin-2 and interleukin-12 in a DNA vaccine formulation. Vaccine 21 (2003) 2882–2889.
Zhang, Y., Taylor, M., Johansen, M. and Bickle, Q. Vaccination of mice with a cocktail DNA vaccine induces a Th1-type immune response and partial protection against Schistosoma japonicum infection. Vaccine 20 (2002) 724–730.
Zhao, W., Su, C., Wu, H.W, Hu, X.M., Shen, L., Ji, M.L., Wang, R.Z., Ma, L., Chen, S.Z. and Zhang, Z.S. Studies on immunoprotection in mice after immunization with Schistosoma japonicum fatty acid binding protein (SjFABPc) recombinant protein. Chinese Journal of Zoonoses 18 (2002) 42–44.
Li, L.Z., Shi, Y.E., Jiang, K., Ning, C.X. and Deng, W.W. Study on the immune effect of multianti genic DNA vaccine pBK-Sj26(Sj32)-Sj23 of Schistosoma japonicum. Acta Univ. Med. Tongji. 30 (2001) 404–406.
Liu, S.X., Song, G.C., Xu, Y. X., McManus, D.P. and Hotez, P.J. Progress in the development of a vaccine against Schistosomiasis in China. Int. J. Infect. Dis. 2 (1998) 176–180.
Zhou, S.H., Liu, S.X., Song, G.C., Xu, Y. X. and Sun, W. Protective immunity induced by the full-length cDNA encoding paramyosin of Chinese Schistosoma japonicum. Vaccine 18 (2000) 3196–3204.
Zhu, Y.C., Ren, J.G., Da’dara, A., Harn, D.A., Xu, M., Si, J., Yu, C.X., Liang, Y.S., Ye, P., Yin, X.R., He, W., Xu, Y.L., Gao, Q., and Hua, W.Q. The protective effect of a Schistosoma japonicum Chinese strain 23kDa plasmid DNA vaccine in pigs is enhanced with IL-12. Vaccine 23 (2004) 78–83.
Gan, Y., Shi, Y.E., Bu, L.Y. Zhu, X.H., Ning, C.X. and Zhu, H.G. Immune responses against Schistosoma japonicum after vaccinating mice with a multivalent DNA vaccine encoding integrated membrane protein Sj23 and cytokine interleukin-12. Chin. Med. J. 117 (2004) 1842–1846.
Li, L.Z. and Shi, Y.E. The protection of Sj23 DNA vaccine on mice. Chin. J. Schisto. Control. 11 (1999) 71–74.
Zhu, X.H. and Shi, Y.E. The Immunoprotective Effect of Co-inoculation of Schistosoma japonicum Fatty Acid Binding Protein (Sj14FABP) DNA and IL-12 in the Mouse. Herald of Medicine 24 (2005) 268–270.
Zhu, X.H., Shi, Y.E., Hu P., Ning, C.X. and Zhu, H.G. Protective immunity induced by plasmid DNA encoding fatty acid binding protein of Schistosoma japonicum in mice. Chin. J. Schisto. Control 17 (2005) 4–8.
Yu, C.X., Zhu, Y.C., Yin, X.R., Ren, J.G., Si, J., Xu, Y.L., Shen L.N. and He, W. Protective immunity induced by the nucleic acid vaccine of fatty acid binding protein of Schistosoma japonicum Chinese strain (SjCFABP) in BALB/c mice. Chin. J. Schisto. Control 14 (2002) 163–167.
Jankovic, D., Wynn, T.A., Kullberg, M.C., Hieny, S., Caspar, P., James, S., Cheever A.W. and Sher, A. Optimal vaccination against Schistosoma mansoni requires the induction of both B cell-and IFN-gamma-dependent effect mechanisms. J. Immunol. 162 (1999) 345–351.
Wynm, T.A. and Hoffmanm, K.F. Defining a schistosomiasis vaccination strategy-is it really Th1 versus Th2? Parasitol. Today 16 (2000) 497–501.
Becker, M.M., Kalinna, B.H., Waine, G.J., and McManus, D.P. Gene cloning, overproduction and purification of a functionally active cytoplasmic fatty acid-binding protein (SjFABPc) from the human blood fluke Schistosoma japonicum. Gene 148 (1994) 321–325.
Gobert, G.N., Stenzel, D.J., Jones, M.K., and McManus, D.P. Immunolocalization of the fatty acid-binding protein Sj FABPc within adult Schistosoma japonicum. Parasitology 115 (1997) 33–39.
Wright, M.D., Melder, A.M., Davern, K.M. and Mitchell, G.F. Serologic activities of the 23 kDa integral membrane proteins of schistosomes. J. Immunol. 147 (1991) 4338–4342.
Davern, K.M., Wright, M.D., Herrmann, V.R. and Mitchell, G.F. Further characterisation of the Schistosoma japon-icum protein Sj26, a target antigen of an immunodiagnostic monoclonal antibody. Mol. Biochem. Parasitol. 48 (1991) 67–75.
Wu, Z.D., Lu, Z.Y. and Yu, X.B. Development of a vaccine against Schistosoma japonicum in China: a review. Acta Tropica 96 (2005) 106–116.
Ren, J.G., Zhu, Y.C., Harn, D.A., Yu, C.X., Yin, X.R., Si, J., He, W., Xu, M., Hua, W.Q., and Xu, Y.L. Protective immunity induced with 23kDa membrane protein DNA vaccine of Schistosoma japonicum Chinese strain in infected C57BL/6 mice. Chin. J. Parasitol. Parasit. Dis. 19 (2001) 336–339.
Waine, G.J., Alarcon, J.B., Qiu, C. and McManus, D.P. Genetic immunization of mice with DNA encoding the 23 kDa transmembrane surface protein of Schistosoma japonicum (Sj23) induces antigen-specific immunoglobulin G antibodies. Parasite Immunol. 21 (1999) 377–381.
Verity, C., McManus, D.P. and Brindley, P.J. Cellular responses to Schistosoma japonicum cathepsin D aartic protease. Parasite Immunol. 24 (2002) 363–367.
McManus, D.P. and Bartley, P.B. A vaccine against Asian schistosomiasis. Parasitol. Int. 53 (2004) 163–173.
Demeure, C.E., Rihet, P., Abel, L., Quattara, M., Bourgoirs, A. and Dassein, A.J. Resistance to S. mansoni in humans: influence of IgE/IgG4 in immunity to reinfection after chemotherapy. J. Infect. Dis. 168 (1993) 1000–1008.
Grzych, J.M., De Bont, J., Liu, J., Neyrinck, J.L., Fontaine, J., Vercruysse, J. and Capron, A. Relationship of impairment of schistosome 28-kilodalton glutathione S-transferase (GST) activity to express of immunity to Schistosoma mattheei in calves vaccinated with recombinant Schistosoma bovis 28-kilodalton GST. Infect. Immun. 66 (1998) 1142–1148.
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Li, C., Yu, L., Liu, Z. et al. Schistosoma japonicum: The design and experimental evaluation of a multivalent DNA vaccine. Cell Mol Biol Lett 11, 449–460 (2006). https://doi.org/10.2478/s11658-006-0036-0
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DOI: https://doi.org/10.2478/s11658-006-0036-0