- Open Access
Emulsions of oil from Adenanthera pavonina L. seeds and their protective effect
Cellular & Molecular Biology Lettersvolume 11, pages438–448 (2006)
In our previous study, we developed very stable formulations of submicron oil-in-water emulsions from Adenanthera pavonina L. (family Leguminosae, subfamily Mimosoideae) seed oil, stabilised with soybean lecithin (SPC). Continuing our research, we introduced an additional co-emulsifier, Tween 80, to those formulations in order to decrease the size of the emulsion particles and improve their stability. Formulations with a mean particle size ranging from 43.6 to 306.5 nm and a negative surface charge from −45.3 to −28.5 mV were obtained. Our stability experiments also revealed that most of the tested formulations had a very good degree of stability over a 3-month storage period, both at 4°C and at room temperature.
Since many intravenous injectable drugs exhibit lytic activity against erythrocytes, we examined this activity for the emulsion form of cardol, a natural compound with already proven hemolytic properties. The incorporation of this agent into the emulsion caused an evident decrease in hemolytic activity (97–99%). This highly protective effect, observed against sheep erythrocytes, was independent of both the composition and the particle size of the emulsions used. Our studies suggest that nonionic surfactant/phospholipid-based emulsions containing this edible oil of A. pavonina L. may be useful as an alternative formulation matrix for pharmaceutical, nutritional or cosmetic applications of otherwise membrane-acting components.
Jumaa, M. and Müller, B.W. Lipid emulsions as a novel system to reduce the hemolytic activity of lytic agents: mechanism of the protective effect. Eur. J. Pharm. Sci. 9 (2000) 285–290.
Zarnowski, R., Jaromin, A., Certik, M., Czabany, T., Fontaine, J., Jakubik, T., Iqbal, M.C.M., Grandmougin-Ferjani, A., Kozubek, A. and Pietr, S.J. The oil of Adenanthera pavonina L. seeds and its emulsions. Z. Naturforsch. 59c (2004) 321–326.
Lam, J.M., Pwee, K.H., Sun, W.Q., Chua, Y.L. and Wang, X.J. Enzyme-stabilizing activity of seed trypsin inhibitors during desiccation. Plant Sci. 142 (1999) 209–218.
Santos, I.S., Da Cunha, M., Machado, O.L.T. and Gomes, V.M. A chitinase from Adenanthera pavonina L. seeds: purification, characterisation and immunolocalisation. Plant Sci. 167 (2004) 1203–1210.
Ali, M.S., Ahmed, F., Azhar, I. and Pervez, M.K. Pavonin: a new five-membered lactone from Advenanthera pavonina Linn. (Mimoaceae). Nat. Prod. Res. 19 (2005) 37–40.
Olajide, O.A., Echianu, C.A., Adedapo, A.D. and Makinde, J.M. Anti-inflammatory studies on Adenanthera pavonina seed extract. Inflammopharmacology 12 (2004) 196–202.
Burkill, I.H. A Dictionary of the Economic Products of the Malay Peninsula. 2nd edition., vol. 1, A-H. Government of Malaysia and Singapore, Kuala Lumpur, 1966.
Kan, P., Chen, Z.B., Lee, C.J. and Chu, I.M. Development of nonionic surfactant/phospholipid o/w emulsion as a paclitaxel delivery system. J. Control. Release 58 (1999) 271–278.
Lundberg, B.B., Risovic, V., Ramaswamy, M. and Wasan, K.M. A lipophilic paclitaxel derivative incorporated in a lipid emulsion for parenteral administration. J. Control. Release 86 (2003) 93–100.
Kang, B.K., Chon, S.K., Kim, S.H., Jeong, S.Y., Kim, M.S., Cho, S.H., Lee, H.B. and Khang, G. Controlled release of paclitaxel from microemulsion containing PLGA and evaluation of anti-tumor activity in vitro and in vivo. Int. J. Pharm. 286 (2004) 147–156.
Kim, S.J., Choi, H.K. and Lee, Y.B. Pharmacokinetic and pharmacodynamic evaluation of cyclosporin A O/W-emulsion in rats. Int. J. Pharm. 249 (2002) 149–156.
Klang, S.H., Baszkin, A. and Benita, S. The stability of piroxicam incorporated in a positively-charged submicron emulsion for ocular administration. Int. J. Pharm. 132 (1996) 33–44.
Tamilvanan, S. and Benita, S. The potential of lipid emulsion for ocular delivery of lipophilic drugs. Eur. J. Pharm. Biopharm. 58 (2004) 357–368.
Pape, W.J.W., Pfannenbecker, U. and Hoppe, U. Validation of the red blood cell test system as in vitro assay for the rapid screening of irritation potential of surfactants. Mol. Toxicol. 1 (1987) 525–536.
Kozubek, A. and Tyman J.H. Resorcinolic lipids, the natural non-isoprenoid phenolic amphiphiles and their biological activity. Chem. Rev. 99 (1999) 1–26.
Kozubek, A. Isolation of 5-n-alkyl-, 5-n-alkenyl-and 5-n-alkdienyl-resorcinol homologs from rye grains. Acta Aliment. Polon. 9 (1985) 185–198.
Zarnowski, R. and Kozubek, A. Alkylresorcinol homologs in Pisum sativum L. varieties. Z. Naturforsch. 54c (1999) 44–48.
Weingarten, C., Magahaes, N.S.S., Baszkin, A., Benita, S. and Seiller, M. Interaction of non-ionic ABA copolymer surfactant with phospholipid monolayers: possible relevance to emulsion stabilization. Int. J. Pharm. 75 (1991) 171–179.
Jumaa, M. and Müller B.W. Influence of non-ionic surfactant PEG-660-12-hydroxy stearate on the surface properties of phospholipid monolayers and their effect on lipid emulsion stability. Col. Polym. Sci. 277 (1999) 347–353.
Jumaa, M. and Müller, B.W. Physicochemical properties of chitosan-lipid emulsions and their stability during the autoclaving process. Int. J. Pharm. 183 (1999) 175–184.
Chung, H., Kim, T.W., Kwon, M., Kwon, I.C. and Jeong, S.Y. Oil components modulate physical characteristics and function of the natural oil emulsions as drug or gene delivery system. J. Control. Release 71 (2001) 339–350.
Yamaguchi, T., Nishizaki, K., Itai, S., Hayashi, H. and Ohshima, H. Physicochemical characterization of parenteral lipid emulsion: influence of cosurfactants on flocculation and coalescence. Pharm. Res. 12 (1995) 1273–1278.
Jumaa, M. and Müller, B.W. In vitro investigation of the effect of various isotonic substances in parenteral emulsions on human erythrocytes. Eur. J. Pharm. Sci. 9 (1999) 207–212.
Nagasaka, Y. and Ishii, F. Interaction between erythrocytes from three different animals and emulsions prepared with various lecithins and oils. Col. Surf. B: Biointerfaces 22 (2001) 141–147.
Sznitowska, M., Dabrowska E.A. and Janicki, S. Solubilizing potential of submicron emulsions and aqueous dispersions of lecithin. Int. J. Pharm. 246 (2002) 203–206.
Kan, P., Chen, Z.B., Kung, R.Y., Lee, C.J., and Chu, I.M. Study on the formulation of o/w emulsion as carriers for lipophilic drugs, Col. Surf. B: Biointerfaces 15 (1999) 117–125.
Liu, F. and Liu, D. Amphipathic polyethylene glycol stabilized emulsions (o/w): physical characterization and in vivo distribution. Int. J. Pharm. 125 (1995) 73–80.
Lundberg, B.B., Mortimer, B.C. and Redgrave, T.G. Submicron lipid emulsions containing amphipathic polyethylene glycol for use as drug-carriers with prolonged circulation time. Int. J. Pharm. 134 (1996) 119–127.