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Emulsions of oil from Adenanthera pavonina L. seeds and their protective effect

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Abstract

In our previous study, we developed very stable formulations of submicron oil-in-water emulsions from Adenanthera pavonina L. (family Leguminosae, subfamily Mimosoideae) seed oil, stabilised with soybean lecithin (SPC). Continuing our research, we introduced an additional co-emulsifier, Tween 80, to those formulations in order to decrease the size of the emulsion particles and improve their stability. Formulations with a mean particle size ranging from 43.6 to 306.5 nm and a negative surface charge from −45.3 to −28.5 mV were obtained. Our stability experiments also revealed that most of the tested formulations had a very good degree of stability over a 3-month storage period, both at 4°C and at room temperature.

Since many intravenous injectable drugs exhibit lytic activity against erythrocytes, we examined this activity for the emulsion form of cardol, a natural compound with already proven hemolytic properties. The incorporation of this agent into the emulsion caused an evident decrease in hemolytic activity (97–99%). This highly protective effect, observed against sheep erythrocytes, was independent of both the composition and the particle size of the emulsions used. Our studies suggest that nonionic surfactant/phospholipid-based emulsions containing this edible oil of A. pavonina L. may be useful as an alternative formulation matrix for pharmaceutical, nutritional or cosmetic applications of otherwise membrane-acting components.

Abbreviations

H:

hemolysis

PI:

polydispersity index

SPC:

soybean lecithin

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Correspondence to Anna Jaromin.

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Jaromin, A., Żarnowski, R. & Kozubek, A. Emulsions of oil from Adenanthera pavonina L. seeds and their protective effect. Cell Mol Biol Lett 11, 438–448 (2006) doi:10.2478/s11658-006-0037-z

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Key words

  • Adenanthera pavonina L
  • Submicron oil-in-water emulsion
  • Hemolysis
  • Cardol