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A comparative gene-expression analysis of CD34+ hematopoietic stem and progenitor cells grown in static and stirred culture systems

Cellular & Molecular Biology Letters volume 11, pages 475–487 (2006)Cite this article

An Erratum to this article was published on 19 September 2006

Abstract

Static and stirred culture systems are widely used to expand hematopoietic cells, but differential culture performances are observed between these systems. We hypothesize that these differential culture outcomes are caused by the physiological responses of CD34+ hematopoietic stem and progenitor cells (HSPCs) to the different physical microenvironments created in these culture devices. To understand the genetic changes provoked by culture microenvironments, the gene expression profiling of CD34+ HSPCs grown in static and stirred culture systems was compared using SMART-PCR and cDNA arrays. The results revealed that 103 and 99 genes were significantly expressed in CD34+ cells from static and stirred systems, respectively. Of those, 91 have similar levels of expression, while 12 show differential transcription levels. These differentially expressed genes are mainly involved in anti-oxidation, DNA repair, apoptosis, and chemotactic activity. A quantitative molecular understanding of the influences of growth microenvironments on transcriptional events in CD34+ HSPCs should give new insights into optimizing culture strategies to produce hematopoietic cells.

Abbreviations

CB:

cord blood

CFC:

colony-forming cells

EDTA:

ethylenediaminetetraacetic acid

HSPCs:

hematopoietic stem and progenitor cells

IMDM:

Iscove’s modified Dulbecco’s medium

MNC:

mononuclear cells

PBS:

phosphate buffer solution

ROS:

reactive oxygen species

SDS:

sodium dodecylsulfate

SSC:

sodium chloride/sodium citrate

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Authors and Affiliations

  1. The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, P.R. China

    Qunliang Li, Qiwei Liu, Haibo Cai & Wen-Song Tan

  2. School of Chemistry & Chemical Engineering, Guangxi University, 100 Daxue Road, Nanning, 530004, P.R. China

    Qunliang Li

Authors
  1. Qunliang Li
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  2. Qiwei Liu
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  3. Haibo Cai
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  4. Wen-Song Tan
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Correspondence to Wen-Song Tan.

Additional information

An erratum to this article is available athttp://dx.doi.org/10.2478/s11658-006-0051-1.

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Li, Q., Liu, Q., Cai, H. et al. A comparative gene-expression analysis of CD34+ hematopoietic stem and progenitor cells grown in static and stirred culture systems. Cell Mol Biol Lett 11, 475–487 (2006). https://doi.org/10.2478/s11658-006-0039-x

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  • DOI: https://doi.org/10.2478/s11658-006-0039-x

Key words

  • CD34+ hematopoietic stem and progenitor cells
  • Ex vivo expansion
  • Culture microenvironment
  • SMART-PCR
  • cDNA array

Cellular & Molecular Biology Letters

ISSN: 1689-1392

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