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AtGRP7 is involved in the regulation of abscisic acid and stress responses in arabidopsis
Cellular & Molecular Biology Letters volume 11, pages 526–535 (2006)
The Arabidopsis AtGRP7 gene, encoding a glycine-rich RNA-binding protein, has been shown to be involved in the regulation of a circadian-regulated negative feedback loop. However, little is known about the role of AtGRP7 in mediating abscisic acid (ABA) and stress responses. Here, we show that AtGRP7 plays a role in both. AtGRP7 was repressed by ABA, high salt and mannitol. Disruption of AtGRP7 by T-DNA insertion led to hypersensitive responses to ABA in both seed germination and root growth assays. The atgrp7-1 mutant was also hypersensitive to osmotic stress conditions, such as high salt and high concentrations of mannitol. In addition, the atgrp7-1 mutant plants accumulated significantly higher transcript levels of two ABA-and stress-inducible genes, RD29A and RAB18, compared with the wild-type plants. Taken together, these results suggest that AtGRP7 is involved in the regulation of ABA and stress responses.
glycine-rich RNA-binding protein
Murashige and Skoog
Fedoroff, N.V. Cross-talk in abscisic acid signaling. Sci. STKE RE10 (2002) 1–12.
Finkelstein, R.R., Gampala, S.S.L. and Rock, C.D. Abscisic acid signaling in seeds and seedlings. Plant Cell (Suppl). 14 (2002) S15–S45.
Himmelbach, A., Yang, Y. and Grill, E. Relay and control of abscisic acid signaling. Curr. Opin. Plant Biol. 6 (2003) 470–479.
Giraudat, J. Abscisic acid signaling. Curr. Opin. Cell Biol. 7 (1995) 232–238.
Pandey, G.K., Cheong, Y.H., Kim, K.N., Grant, J.J., Li, L., Hung, W., D’Angelo, C., Weinl, S., Kudla, J. and Luan, S. The calcium sensor calcineurin B-like 9 modulates abscisic acid sensitivity and biosynthesis in Arabidopsis. Plant Cell. 16 (2004) 1912–1924.
Kim, K.N., Cheong, Y.H., Grant, J.J., Pandey, G.K. and Luan, S. CIPK3, a calcium sensor-associated protein kinase that regulates abscisic acid and cold signal transduction in Arabidopsis. Plant Cell. 15 (2003) 411–423.
Sridha, S. and Wu, K. Identification of AtHD2C as a novel regulator of abscisic acid responses in Arabidopsis. Plant J. 46 (2006) 124–133.
Pandey, G.K., Grant, J.J., Cheong, Y.H., Kim, B.G., Li, L. and Luan, S. ABR1, an APETALA2-domain transcription factor that functions as a repressor of ABA response in Arabidopsis. Plant Physiol. 139 (2005) 1185–1193.
Razem, F.A., El-Kereamy, A., Abrams S.R. and Hill R.D. The RNA-binding protein FCA is an abscisic acid receptor. Nature 439 (2006) 290–294.
Sato, N. A cold-regulated cyanobacterial gene cluster encodes RNAbinding protein and ribosomal protein S21. Plant Mol. Biol. 24 (1994) 819–823.
Sato, N. A family of cold-regulated RNA-binding protein genes in the cyanobacterium Anabaena variabilis M3. Nucleic. Acids Res. 23 (1995) 2161–2167.
Gómez, J., Sánchez, M.D., Stiefel, V., Rigau, J., Puigdomènech, P. and Pagès, M. A gene induced by the plant hormone abscisic acid in response to water stress encodes a glycine-rich protein. Nature 334 (1988) 262–264.
Bergeron, D., Beauseigle, D. and Bellemare, G. Sequence and expression of a gene encoding a protein with RNA-binding and glycine-rich domains in Brassica napus. Biochim. Biophys. Acta. 1216 (1993) 123–125.
Hirose, T., Sugita, M. and Sugiura, M. cDNA structure, expression and nucleic acid-binding properties of three RNA-binding proteins in tobacco: occurrence of tissue-specific alternative splicing. Nucleic. Acids Res. 21 (1993) 3981–3987.
Carpenter, C.D., Kreps, J.A. and Simon, A.E. Genes encoding glycinerich Arabidopsis thaliana proteins with RNA-binding motifs are influenced by cold treatment and an endogenous circadian rhythm. Plant Physiol. 104 (1994) 1015–1025.
Derry, J.M., Kerns, J.A. and Francke, U. RBM3, a novel human gene Xp11.23 with putative RNA-binding domain. Hum. Mol. Genet. 4 (1995) 2307–2311.
Kwak, K.J., Kim, Y.O. and Kang, H. Characterization of transgenic Arabidopsis plants overexpressing GR-RBP4 under high salinity, dehydration, or cold stress. J. Exp. Bot. 56 (2005) 3007–3016.
Heintzen, C., Nater, M., Apel, K. and Staiger, D. AtGRP7, a nuclear RNA-binding protein as a component of a circadian-regulated negative feedback loop in Arabidopsis thaliana. Proc. Natl. Acad. Sci. USA. 94 (1997) 8515–8520.
Murashige, T. and Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant 15 (1962) 473–497.
Alonso, J.M., Stepanova, A.N., Leisse, T.J., Kim, C.J., Chen, H., Shinn, P., Stevenson, D.K., Zimmerman, J., Barajas, P., Cheuk, R. Gadrinab, C., Heller, C., Jeske, A., Koesema, E., Meyers, C.C., Parker, H., Prednis, L., Ansari, Y., Choy, N., Deen, H., Geralt, M., Hazari, N., Hom, E., Karnes, M., Mulholland, C., Ndubaku R., Schmidt, I., Guzman, P., Aguilar-Henonin, L., Schmid, M., Weigel, D., Carter, D.E., Marchand, T., Risseeuw, E., Brogden, D., Zeko, A., Crosby, W.L., Berry, C.C. and Ecker J.R. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301 (2003) 653–657.
Staiger, D. and Apel, K. Circadian clock-regulated expression of an RNA-binding protein in Arabidopsis: characterisation of a minimal promoter element. Mol. Gen. Genet. 261 (1999) 811–819.
Zimmermann, P., Hirsch-Hoffmann, M., Hennig, L. and Gruissem, W. GENEVESTIGATOR. Arabidopsis microarray database and analysis toolbox. Plant Physiol. 136 (2004) 2621–2632.
Rock, C.D. Pathways to abscisic acid-regulated gene expression. New Phytol. 148 (2000) 357–396.
Leung, J. and Giraudat, J. Abscisic acid signal transduction. Annu. Rev. Plant Physiol. Plant Mol. Biol. 49 (1998) 199–222.
Nishiyama, H., Itoh, K., Kaneko, Y., Kishishita, M., Yoshida, O. and Fujita, J. A glycine-rich RNA-binding protein mediating cold-inducible suppression of mammalian cell growth. J. Cell Biol. 137 (1997) 899–908.
Seo, M. and Koshiba, T. Complex regulation of ABA biosynthesis in plants. Trends Plant Sci. 7 (2002) 41–48.
Zeevaart, J.A.D. and Creelman, R.A. Metabolism and physiology of abscisic acid. Annu. Rev. Plant Physiol. Plant Mol. Biol. 39 (1988) 439–473.
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Cao, S., Jiang, L., Song, S. et al. AtGRP7 is involved in the regulation of abscisic acid and stress responses in arabidopsis. Cell Mol Biol Lett 11, 526–535 (2006). https://doi.org/10.2478/s11658-006-0042-2
- AtGRP7 gene
- Abscisic acid
- Osmotic stress