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AtGRP7 is involved in the regulation of abscisic acid and stress responses in arabidopsis

Abstract

The Arabidopsis AtGRP7 gene, encoding a glycine-rich RNA-binding protein, has been shown to be involved in the regulation of a circadian-regulated negative feedback loop. However, little is known about the role of AtGRP7 in mediating abscisic acid (ABA) and stress responses. Here, we show that AtGRP7 plays a role in both. AtGRP7 was repressed by ABA, high salt and mannitol. Disruption of AtGRP7 by T-DNA insertion led to hypersensitive responses to ABA in both seed germination and root growth assays. The atgrp7-1 mutant was also hypersensitive to osmotic stress conditions, such as high salt and high concentrations of mannitol. In addition, the atgrp7-1 mutant plants accumulated significantly higher transcript levels of two ABA-and stress-inducible genes, RD29A and RAB18, compared with the wild-type plants. Taken together, these results suggest that AtGRP7 is involved in the regulation of ABA and stress responses.

Abbreviations

ABA:

abscisic acid

GR-RBP:

glycine-rich RNA-binding protein

MS:

Murashige and Skoog

TFs:

transcription factors

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Correspondence to Shuqing Cao.

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Cao, S., Jiang, L., Song, S. et al. AtGRP7 is involved in the regulation of abscisic acid and stress responses in arabidopsis. Cell Mol Biol Lett 11, 526–535 (2006). https://doi.org/10.2478/s11658-006-0042-2

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Key words

  • AtGRP7 gene
  • Abscisic acid
  • Osmotic stress