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Helicobacter pylori infection can modulate the susceptibility of gastric mucosa cells to MNNG

Cellular & Molecular Biology Letters volume 11, pages570–578(2006)Cite this article

Abstract

The pathogenesis of stomach cells can be associated with their susceptibility to exogenous dietary irritants, like nitrosamines such as dimethylnitrosamines (DMNA), and to the effects of non-dietary factors, including Helicobacter pylori infection. We used N-methyl-N’-nitro N-nitrosoguanidyne (MNNG) as a surrogate agent that induces a spectrum of DNA damage similar to DMNA. Using the alkaline comet assay, we showed that antioxidants — vitamins C and E, quercetin, and melatonin — reduced the genotoxic effect of MNNG in H. pylori-infected and non-infected human gastric mucosa cells (GMCs). To compare the sensitivity of the stomach and the blood, the experiment was also carried out in peripheral blood. We observed a higher level of DNA damage induced by MNNG in H. pylori-infected than in noninfected GMCs. We did not note any difference in the efficacy of the repair of the damage in either type of GMC. H. pylori infection may play an important role in the pathogenesis of GMCs, as it can modulate their susceptibility to dietary mutagens/carcinogens, thus contributing to gastric cancer.

Abbreviations

GMCs:

gastric mucosa cells

MNNG:

N-methyl-N’-nitro-N-nitrosoguanidine

PBL:

peripherial blood lymphocytes

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Affiliations

  1. Department of Molecular Genetics, University of Łódź, Banacha 12/16, 90-237, Łódź, Poland

    Michał Arabski & Janusz Błasiak

  2. Outpatient Gastroenterology Department, St John of God’s Hospital, Kosynierów Gdyńskich 61, 93-357, Łódź, Poland

    Paweł Kazmierczak

  3. Department of Gastroenterology and Internal Diseases, Medical University, 90-647, Łódź, Plac Hallera 1, Łódź, Poland

    Maria Wiśniewska-Jarosińska, Grażyna Klupińska & Jan Chojnacki

  4. N. Copernicus Hospital, Pabianicka, Łódź, Poland

    Zbigniew Morawiec

  5. Department of Head and Neck Cancer, Medical University of Łódź, Łódź, Poland

    Zbigniew Morawiec

  6. Depatment of Diabeteology and Internal Diseases, Medical University of Łódź, Rewolucji 1905 37/39, 90-214, Łódź, Poland

    Alina Morawiec-Bajda & Józef Drzewoski

Authors
  1. Michał Arabski
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  2. Paweł Kazmierczak
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  3. Maria Wiśniewska-Jarosińska
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  4. Zbigniew Morawiec
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  5. Alina Morawiec-Bajda
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  6. Grażyna Klupińska
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  7. Józef Drzewoski
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  8. Jan Chojnacki
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  9. Janusz Błasiak
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Corresponding author

Correspondence to Janusz Błasiak.

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Arabski, M., Kazmierczak, P., Wiśniewska-Jarosińska, M. et al. Helicobacter pylori infection can modulate the susceptibility of gastric mucosa cells to MNNG. Cell Mol Biol Lett 11, 570–578 (2006). https://doi.org/10.2478/s11658-006-0045-z

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Key words

  • Helicobacter pylori
  • DNA damage
  • DNA repair
  • MNNG
  • Melatonin
  • Vitamin C
  • Vitamin E
  • Quercetin

Cellular & Molecular Biology Letters

ISSN: 1689-1392

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