- Open Access
Helicobacter pylori infection can modulate the susceptibility of gastric mucosa cells to MNNG
Cellular & Molecular Biology Letters volume 11, pages570–578(2006)
The pathogenesis of stomach cells can be associated with their susceptibility to exogenous dietary irritants, like nitrosamines such as dimethylnitrosamines (DMNA), and to the effects of non-dietary factors, including Helicobacter pylori infection. We used N-methyl-N’-nitro N-nitrosoguanidyne (MNNG) as a surrogate agent that induces a spectrum of DNA damage similar to DMNA. Using the alkaline comet assay, we showed that antioxidants — vitamins C and E, quercetin, and melatonin — reduced the genotoxic effect of MNNG in H. pylori-infected and non-infected human gastric mucosa cells (GMCs). To compare the sensitivity of the stomach and the blood, the experiment was also carried out in peripheral blood. We observed a higher level of DNA damage induced by MNNG in H. pylori-infected than in noninfected GMCs. We did not note any difference in the efficacy of the repair of the damage in either type of GMC. H. pylori infection may play an important role in the pathogenesis of GMCs, as it can modulate their susceptibility to dietary mutagens/carcinogens, thus contributing to gastric cancer.
gastric mucosa cells
peripherial blood lymphocytes
Yamaguchi, N. and Kakizoe, T. Synergistic interaction between Helicobacter pylori gastritis and diet in gastric cancer. Lancet 2 (2001) 84–94.
Zatomski, W., Oshima, H. and Przewoźniak, K. Urinary excretion of N-nitrosamino acids and nitrate by inhabitants of high-and low risk areas for stomach cancer in Poland. Int. J. Cancer 44 (1989) 823–827.
Mannick, E.E., Bravo, L.E., Zarama, G., Realpe, J.L., Zhang, X.J., Ruiz, B., Fontham, E.T., Mera, R., Miller, M.J. and Correa, P. Inducible nitric oxide synthase, nitrotyrosine, and apoptosis in Helicobacter pylori gastritis: effect of antibiotics and antioxidants. Cancer Res. 56 (1996) 3238–3243.
Parsonnet, J., Friedmann, G.D., Vandersteen, D.P., Chang, Y., Vogelman, J., Orentreich, N. and Sibley, R.K. Helicobacter pylori infection and the risk of gastric carcinoma. N. Engl. J. Med. 325 (1991) 1127–1131.
Eurogast Study Group. An international association between Helicobacter pylori infection and gastric cancer. Lancet 341 (1993) 1359–1362s.
De Luca, A. and Iaquinto, G. Helicobacter pylori and gastric diseases: a dangerous association. Cancer Lett. 213 (2004) 1–10.
Blasiak, J., Trzeciak, A., Gasiorowska, A., Drzewoski, J. and Malecka-Panas, E. Vitamin C and quercetin modulate DNA-damaging effect of N-methyl-N’-nitro-N-nitrosoguanidine (MNNG). Plant Foods Hum. Nutr. 57 (2002) 53–61.
Singh, N.P., McKoy, M.T., Tice, R.R. and Schneider, E.L. A simple technique for quanitation of low levels of damage in individual cells. Exp. Cell Res. 175 (1988) 184–191.
Arabski, M., Kazmierczak, P., Wisniewska-Jarosinska, P., Poplawski, T., Klupinska, G., Chojnacki, J., Drzewoski, J. and Blasiak, J. Interaction of amoxicillin with DNA in human lymphocytes and H. pylori-infected and non-infected gastric mucosa cells. Chem-Biol. Interact. 152 (2005) 13–24.
Bignami, M., O’Driscoll, M., Aquilina, G. and Karran, P. Unmasking a killer: DNA O(6)-methylguanine and the cytotoxicity of methylating agents. Mutat. Res. 462 (2000) 71–82.
Arabski, M., Klupinska, G., Chojnacki, J., Kazmierczak, P., Wisniewska-Jarosinska, M., Drzewoski, J. and Blasiak, J. DNA damage and repair in Helicobacter pylori-infected gastric mucosa cells. Mutat. Res. 570 (2005) 129–135.
Kimura, N. and Miwa, T. Immunocytochemical and biochemical studies of glutathione-peroxidase (GSH-PO) in rat gastric parietal cells. Acta Histochem. Cytochem. 35 (2002) 23–31.
Figura, N. Helicobacter pylori factors involved in the development of gastroduodenal mucosal damage and ulceration. J. Clin. Gastroenterol. 1 (1997) 149–163.
Reiter, R.J., Tan, D.X., Manchester, L.C. and Qi, W. Biochemical reactivity of melatonin with reactive oxygen and nitrogen species. Cell Biochem. Biophys. 34 (2001) 237–256.
Liu, J., Li, X., Yue, Y., Li, J., He, T. and He, Y. The inhibitory effect of quercetin on IL-6 production by LPS-stimulated neutrophils. Cell Mol. Immunol. 2 (2005) 455–460.
Kempuraj, D., Madhappan, B., Christodoulou, S., Boucher, W., Cao, J., Papadopoulou, N., Cetrulo, C.L. and Theoharides, T.C. Flavonols inhibit proinflammatory mediator release, intracellular calcium ion levels and protein kinase C theta phosphorylation in human mast cells. Br. J. Pharmacol. 145 (2005) 934–944.
Everett, S.M., Drake, I.M., White, K.L.M., Mapstone, N.P., Chalmers, D.M., Schorah, J. and Axon, A.T.R. Antioxidant vitamin supplement do not reduce reactive oxygen species activity in Helicobacter pylori gastritis in the short term. Br. J. Nutr. 87 (2002) 3–11.
Honig, A., Witte, F., Mirecka, J., Binder, C. and Schauer, A. Helicobacter pylori-induced hyperproliferation: relevance for gastric cancer development in connection with mutagenic factors. Anticancer Res. 20 (2000) 1641–1648.
About this article
Cite this article
Arabski, M., Kazmierczak, P., Wiśniewska-Jarosińska, M. et al. Helicobacter pylori infection can modulate the susceptibility of gastric mucosa cells to MNNG. Cell Mol Biol Lett 11, 570–578 (2006). https://doi.org/10.2478/s11658-006-0045-z
- Helicobacter pylori
- DNA damage
- DNA repair
- Vitamin C
- Vitamin E