- Open Access
Genetic diversity of SIRE-1 retroelements in annual and perennial glycine species revealed using SSAP
- Catherine Chesnay1,
- Amar Kumar1 and
- Stephen R. Pearce2Email author
https://doi.org/10.2478/s11658-006-0054-y
© University of Wrocław 2006
- Received: 11 July 2006
- Accepted: 6 October 2006
- Published: 13 November 2006
Abstract
Sequence Specific Amplification Polymorphisms (SSAP) were used to measure the distribution and structure of SIRE-1 retroelement populations in annual and perennial Glycine species. For SSAP analysis, primers corresponding to a region immediately upstream of the 3’LTR of the soybean retroelement SIRE-1 were chosen. Analysis reveals that SIRE-1 is present throughout the Glycine genus and shows that the annual species have similar SIRE-1 populations whilst the perennial species have much more distinct and diverse populations. The high number of species-specific subgroups suggest that SIRE-1 has been active and evolving independently in each species during the course of Glycine evolution.
Key words
- Glycine
- Retroelement
- SSAP
- phylogeny
- SIRE-1
- copia