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Genetic diversity of SIRE-1 retroelements in annual and perennial glycine species revealed using SSAP

Abstract

Sequence Specific Amplification Polymorphisms (SSAP) were used to measure the distribution and structure of SIRE-1 retroelement populations in annual and perennial Glycine species. For SSAP analysis, primers corresponding to a region immediately upstream of the 3’LTR of the soybean retroelement SIRE-1 were chosen. Analysis reveals that SIRE-1 is present throughout the Glycine genus and shows that the annual species have similar SIRE-1 populations whilst the perennial species have much more distinct and diverse populations. The high number of species-specific subgroups suggest that SIRE-1 has been active and evolving independently in each species during the course of Glycine evolution.

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Correspondence to Stephen R. Pearce.

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Chesnay, C., Kumar, A. & Pearce, S.R. Genetic diversity of SIRE-1 retroelements in annual and perennial glycine species revealed using SSAP. Cell Mol Biol Lett 12, 103–110 (2007). https://doi.org/10.2478/s11658-006-0054-y

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  • DOI: https://doi.org/10.2478/s11658-006-0054-y

Key words

  • Glycine
  • Retroelement
  • SSAP
  • phylogeny
  • SIRE-1
  • copia