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Prins and C-PRINS: Promising tools for the physical mapping of the lupin genome

Cellular & Molecular Biology Letters volume 12, pages 16–24 (2007)Cite this article

Abstract

Two molecular cytogenetics methods, PRINS (primed in situ DNA labeling) and C-PRINS (cycling PRINS), were optimized for the physical mapping of several types of DNA sequences on the mitotic chromosomes of the narrow-leafed lupin (Lupinus angustifolius L.). The fragment of the FokI element from Vicia faba was localised by indirect PRINS reaction. Two other sequences, fragments of the coding sequences of L. luteus and of L. angustifolius, were localised by indirect C-PRINS. These techniques are faster and more sensitive than FISH, and they allowed the mapping of short DNA fragments. The data obtained shows that both types of PRINS are valuable tools for chromosome identification in lupin.

Abbreviations

BAC:

bacterial artificial chromosome

C-PRINS:

cycling PRINS

DAPI:

4′,6′-diamidino-2-phenylindole

FISH:

fluorescence in situ hybridization

PCR:

polymerase chain reaction

PRINS:

primed in situ DNA labeling

References

  1. Atkins, C.A., Smith, P.M.C., Gupta, S., Jones, M.G.K. and Caligari, P.D.S. Genetics, cytology and biotechnology. In: Lupins as crop plants: biology, production and utilization (Gladstones J.S., Atkins C.A., Hamblin J., Eds). CAB International (1998) 67–92.

  2. Naganowska, B. and ładoń, D. Chromosomes of Lupinus hispanicus subsp. hispanicus Boiss. et Reut., L. luteus L. and their hybrids. J. Appl. Genet. 41 (2000) 167–170.

    Google Scholar 

  3. Naganowska, B. and Zielińska, A. Physical mapping of 18S-25S rDNA and 5S rDNA in Lupinus via fluorescent in situ hybridization. Cell. Mol. Biol. Lett.. 7 (2002) 665–670.

    PubMed  CAS  Google Scholar 

  4. Naganowska, B., Doležel, J. and Święcicki, W.K. Development of molecular cytogenetics and physical mapping of ribosomal genes in Lupinus. Biol. Plant. 46 (2003) 211–215.

    Article  CAS  Google Scholar 

  5. Naganowska, B. and Kaczmarek, A. Localisation of rDNA in the Lupinus genome during the cell cycle. J. Appl. Genet. 45 (2004) 189–193.

    PubMed  Google Scholar 

  6. Hajdera, I., Siwinska, D., Hasterok, R. and Maluszynska, J. Molecular cytogenetic analysis of genome structure in Lupinus angustifolius and Lupinus cosentinii. Theor. Appl. Genet. 107 (2003) 988–996.

    Article  PubMed  CAS  Google Scholar 

  7. Schwarzacher, T., Leicht, A.R. and Heslop-Harrison J.S. DNA:DNA in situ hybridization — methods for light microscopy. Plant Cell Biology: A Practical Approach. (Harris, N., Oparka, K.J. Eds.) Oxford, Oxford University Press (1994) 1–20.

    Google Scholar 

  8. Koch, J.E., Kolvraa, S., Petersen, K.B., Gregersen, N. and Bolund, L. Oligonucleotide-priming methods for the chromosome-specific labelling of α satellite DNA in situ. Chromosoma 98 (1989) 259–265.

    Article  PubMed  CAS  Google Scholar 

  9. Gosden, J., Hanratty, D., Starling, J., Fantes, J., Mitchell, A. and Porteous, D. Oligonucleotide primed in situ DNA synthesis (PRINS): a method for mapping, banding and investigation of sequence organization. Cytogenet. Cell Genet. 57 (1991) 100–104.

    Article  PubMed  CAS  Google Scholar 

  10. Gosden, J.R Identification and quantitation of human chromosomes by primed in situ synthesis. Chromosome Res. 4 (1996) 331–334.

    Article  PubMed  CAS  Google Scholar 

  11. Pallestor, F., Quennesson, I., Coignet, L., Girardet, A., Andréo, B. and Charlieu, J.P. Direct detection of disomy in human sperm by the PRINS technique. Human Genet. 97 (1996) 21–25.

    Google Scholar 

  12. Abbo, S, Miller, T.E. and King, I.P. Primed-induced in situ hybridization to plant chromosomes. Genome 36 (1993) 815–817.

    PubMed  CAS  Google Scholar 

  13. Mukai, Y. and Appels, R. Direct chromosome mapping of plant genes by in situ polymerase chain reaction (in situ PCR). Chromosome Res. 4 (1996) 401–403.

    PubMed  CAS  Google Scholar 

  14. Menke, M., Fuchs, J. and Schubert, I. A comparison of sequence resolution on plant chromosomes: PRINS versus FISH. Theor. Appl. Genet. 97 (1998) 1314–1320.

    Article  CAS  Google Scholar 

  15. Kubaláková, M., Macas, J. and Doležel, J. Mapping of repeated DNA sequences in plant chromosomes by PRINS and C-PRINS. Theor. Appl. Genet. 94 (1997) 758–763.

    Article  Google Scholar 

  16. Kubaláková, M., Vrána, J., číhalíková, J., Lysák J.and Doležel, J. Localization of DNA sequences on plant chromosomes using PRINS and CPRINS. Methods Cell Sci. 23 (2001) 71–82.

    Article  PubMed  Google Scholar 

  17. Doležel, J., Cíhalíková, J., Weiserová, J. and Lucretti, S. Cell cycle synchronization in plant root meristems. Methods Cell Sci. 21 (1999) 95–107.

    Article  PubMed  Google Scholar 

  18. Nuc, P and Wypijewski, K. EST and cDNA collection from the yellow lupin hypocotyl. http://www.staff.amu.edu.pl/≈ibmib/zbiochemii.

  19. You, M., Boersma, J.G., Buirchell, B.J. Sweetingham, M.W., Siddique, K.H.M. and Yang, H. A PCR-based molecular marker applicable for marker-assisted selection for anthracnose disease resistance in lupin breeding. Cell. Mol. Biol. Lett. 10 (2005) 123–134.

    PubMed  CAS  Google Scholar 

  20. Kato, A., Yakura, K. and Tanifuji, S. Sequence analysis of Vicia faba repeated DNA, the FokI repeat element. Nucleic Acids Res. 12 (1984) 6415–6426.

    PubMed  CAS  Google Scholar 

  21. Kasprzak, A., Šafář, J., Janda, J., Doležel, J., Wolko, B. and Naganowska, B. The bacterial artificial chromosome (BAC) library of the narrow-leafed lupin (Lupinus angustifolius L.). Cell. Mol. Biol. Lett. 11 (2006) 396–407.

    Article  PubMed  CAS  Google Scholar 

  22. Boersma, J.G., Pallotta, M., Li, Ch., Buirchell, B.J., Sivasithamparam, K. and Yang, H. Construction of a genetic linkage map using mflp and identification of molecular markers linked to domestication genes in narrowleafed lupin (Lupinus angustifolius L.). Cell. Mol. Biol. Lett. 10 (2005) 331–344.

    PubMed  CAS  Google Scholar 

  23. Nelson, M.W., Phan, H.T.T., Ellwood, S.R., Moolhuijzen, P.M., Hane, J., Williams, A., O’Lone, C.E., Fosu-Nyarko, J., Scobie, M., Cakir, M., Jones, M.G.K., Bellgard, M., Książkiewicz, M., Wolko, B., Barker, S.J., Oliver, R.P. and Cowling, W.A. The first gene-based map of Lupinus angustifolius L. — location of domestication genes and conserved synteny with Medicago truncatula. Theor. Appl. Genet. (2006) DOI 10.1007/s00122-006-0288-0.

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Authors and Affiliations

  1. Polish Academy of Sciences, Institute of Plant Genetics, Strzeszyńska 34, 60-479, Poznań, Poland

    Anna Kaczmarek, Barbara Naganowska & Bogdan Wolko

Authors
  1. Anna Kaczmarek
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  2. Barbara Naganowska
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  3. Bogdan Wolko
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Kaczmarek, A., Naganowska, B. & Wolko, B. Prins and C-PRINS: Promising tools for the physical mapping of the lupin genome. Cell Mol Biol Lett 12, 16–24 (2007). https://doi.org/10.2478/s11658-006-0056-9

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  • DOI: https://doi.org/10.2478/s11658-006-0056-9

Key words

  • PRINS
  • C-PRINS
  • Physical mapping
  • Chromosomes
  • Lupinus angustifolius L

Cellular & Molecular Biology Letters

ISSN: 1689-1392

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