Open Access

Prins and C-PRINS: Promising tools for the physical mapping of the lupin genome

  • Anna Kaczmarek1,
  • Barbara Naganowska1 and
  • Bogdan Wolko1
Cellular & Molecular Biology LettersAn International Journal200612:56

https://doi.org/10.2478/s11658-006-0056-9

Received: 9 January 2006

Accepted: 25 July 2006

Published: 13 November 2006

Abstract

Two molecular cytogenetics methods, PRINS (primed in situ DNA labeling) and C-PRINS (cycling PRINS), were optimized for the physical mapping of several types of DNA sequences on the mitotic chromosomes of the narrow-leafed lupin (Lupinus angustifolius L.). The fragment of the FokI element from Vicia faba was localised by indirect PRINS reaction. Two other sequences, fragments of the coding sequences of L. luteus and of L. angustifolius, were localised by indirect C-PRINS. These techniques are faster and more sensitive than FISH, and they allowed the mapping of short DNA fragments. The data obtained shows that both types of PRINS are valuable tools for chromosome identification in lupin.

Key words

PRINS C-PRINS Physical mapping Chromosomes Lupinus angustifolius L

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