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Prins and C-PRINS: Promising tools for the physical mapping of the lupin genome

Abstract

Two molecular cytogenetics methods, PRINS (primed in situ DNA labeling) and C-PRINS (cycling PRINS), were optimized for the physical mapping of several types of DNA sequences on the mitotic chromosomes of the narrow-leafed lupin (Lupinus angustifolius L.). The fragment of the FokI element from Vicia faba was localised by indirect PRINS reaction. Two other sequences, fragments of the coding sequences of L. luteus and of L. angustifolius, were localised by indirect C-PRINS. These techniques are faster and more sensitive than FISH, and they allowed the mapping of short DNA fragments. The data obtained shows that both types of PRINS are valuable tools for chromosome identification in lupin.

Abbreviations

BAC:

bacterial artificial chromosome

C-PRINS:

cycling PRINS

DAPI:

4′,6′-diamidino-2-phenylindole

FISH:

fluorescence in situ hybridization

PCR:

polymerase chain reaction

PRINS:

primed in situ DNA labeling

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Kaczmarek, A., Naganowska, B. & Wolko, B. Prins and C-PRINS: Promising tools for the physical mapping of the lupin genome. Cell Mol Biol Lett 12, 16–24 (2007). https://doi.org/10.2478/s11658-006-0056-9

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  • DOI: https://doi.org/10.2478/s11658-006-0056-9

Key words

  • PRINS
  • C-PRINS
  • Physical mapping
  • Chromosomes
  • Lupinus angustifolius L