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A proteomic analysis of the effect of mapk pathway activation on l-glutamate-induced neuronal cell death

Cellular & Molecular Biology Letters volume 12, pages 139–147 (2007)Cite this article

Abstract

Oxidative stress has been implicated in the pathogenesis of neuronal degenerative diseases. It is also widely known that oxidative stress induces mitogen-activated protein kinase (MAPK) signaling cascades. In this study, we used proteomic analysis to investigate the role of the MAPK pathway in oxidative stress-induced neuronal cell death. The results demonstrated that several proteins, including eukaryotic translation elongation factor 2 (eEF2) and enolase I, showed a differential expression pattern during the neuronal cell death process, and this was MAPK pathway dependent. Several chaperone and cytoskeletal proteins including heat shock protein 70, calreticulin, vimentin, prolyl 4-hydroxylase β polypeptide, and transgelin 2 were up-or down-regulated, despite their expressions not depending on the MAPK pathway. These findings strongly suggest that the expressions of proteins which play protective roles are independent of the MAPK pathway. On the other hand, eEF2 and enolase I may be the downstream targets of the MAPK pathway.

Abbreviations

Cy2:

3-(4-carboxymethyl) phenylmethyl-3’-ethyloxacarbocyanine halide

Cy3:

1-(5-carboxypentile)-1’-propylindocarbocyanine halide

Cy5:

1-(5-carboxypentile)-1’-methylindocarbocyanine halide

DIGE:

differential in gel electrophoresis

GSH:

glutathione

MAPK:

mitogen-activated protein kinase

MEK:

MAPK/ERK kinase

ROS:

reactive oxygen species

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Authors and Affiliations

  1. Korean Research Institute of Bioscience and Biotechnology (KRIBB), 52 Eoeun-Dong, Yusung-Gu, Daejeon, 305-333, Republic of Korea

    Sunghyun Kang, Eun Young Kim, Young Jae Bahn, Do Hee Lee, Sung Goo Park, Tae-Sung Yoon, Byoung Chul Park & Kwang-Hee Bae

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  1. Sunghyun Kang
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  2. Eun Young Kim
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  3. Young Jae Bahn
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  4. Jin Woong Chung
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Correspondence to Kwang-Hee Bae.

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Kang, S., Kim, E.Y., Bahn, Y.J. et al. A proteomic analysis of the effect of mapk pathway activation on l-glutamate-induced neuronal cell death. Cell Mol Biol Lett 12, 139–147 (2007). https://doi.org/10.2478/s11658-006-0057-8

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  • DOI: https://doi.org/10.2478/s11658-006-0057-8

Key words

  • Apoptosis
  • HT22
  • MAPK
  • Oxidative stress
  • Reactive oxygen species
  • U0126

Cellular & Molecular Biology Letters

ISSN: 1689-1392

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