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The immunoregulatory effects of edeine analogues in mice

Abstract

The edeines analogs were tested in several in vitro and in vivo assays using the mouse model, with edeine B (peptide W1) and cyclosporine A as reference compounds. The peptides displayed moderate, stimulatory effects on concanavalin A-induced (ConA-induced) splenocyte proliferation, whereas their effects on pokeweed mitogen-induced (PWM-induced) splenocyte proliferation were inhibitory. The peptides inhibited lipopolysacharide-induced (LPS-induced) tumor necrosis factor alpha production but had little effect on interleukin 6 production. In the model of the humoral immune response in vitro to sheep red blood cells, peptide 1 was distinctly stimulatory in the investigated concentrations (1-100 μg/ml), whereas peptides 3 and 4 only stimulated the number of antibody-forming cells at the highest concentration (100 μg/ml). In the model of the delayed type hypersensitivity in vivo to ovalbumin, the peptides were moderately suppressive (3 being the most active). The reference peptide W1 stimulated ConA-induced cell proliferation at 1–10 μg/ml but was inhibitory at 100 μg/ml. It also inhibited PWM-induced cell proliferation in a dose-dependent manner. This peptide had no effect on the humoral immune response in vitro or on cytokine production, but inhibited DTH reaction in vivo. The relationship between structure and activity, and a possible mode of action of the peptides, is discussed in this paper.

Abbreviations

AFC:

antibody-forming cells

cFa:

complete Freund’s adjuvant

Con A:

concanavalin A

CsA:

cyclopsporine

DTH:

delayed type hypersensitivity

iFa:

incomplete Freund’s adjuvant

LPS:

lipopolysaccharide

OD:

optical density

OVA:

ovalbumin

PWM:

pokeweed mitogen

SRBC:

sheep red blood cells

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Correspondence to Michał Zimecki.

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Czajgucki, Z., Zimecki, M. & Andruszkiewicz, R. The immunoregulatory effects of edeine analogues in mice. Cell Mol Biol Lett 12, 149–161 (2007). https://doi.org/10.2478/s11658-006-0061-z

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  • DOI: https://doi.org/10.2478/s11658-006-0061-z

Key Words

  • Edeine
  • Immune response
  • Mice