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Tetraploid somatic hybrids of potato (Solanum tuberosum L.) obtained from diploid breeding lines

Abstract

Intraspecific somatic hybrids between 16 different diploid breeding lines of Solanum tuberosum L. were produced by PEG-induced fusion. Manually selected heterokaryons were cultured in a Millicells-CM using a post-fusion protoplast mixture. Plants were regenerated from calli derived from heterokaryons obtained from 10 out of 38 combinations of diploid lines. Of the tested putative somatic hybrids, 14.2% were diploid, 72.8% were tetraploid and 13% pentaploid. The DNA amplification pattern obtained with RAPD or semi-random primers confirmed that 6 fusion combinations were hybrids. In most cases, the morphological traits were intermediate to those of the diploid fusion partners. About 23.0% of the tested somatic hybrids showed variation in their morphology. Of the tested somatic hybrids, 78.0% flowered and 86.0% tuberized. The cytoplasm of 9 diploid lines and 6 somatic hybrid combinations was analysed. Two of the diploid lines had W/S chloroplasts and α or ε mitochondria; the remainder contained T chloroplasts and β mitochondria. All the analysed somatic hybrids carried T chloroplasts and β mitochondria.

Abbreviations

cpDNA:

chloroplast DNA

CTAB:

cetyltrimethyl ammonium bromide

FDA:

fluorescein diacetate

mtDNA:

mitochondrial DNA

NAA:

naphthaleneaceic acid

PEG:

polyethylene glicol

PVP:

polyvinylpyrrolidone

RAPD:

random amplification of polymorphic DNA

RFLP:

restriction fragments length polymorphism

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Correspondence to Wacław Orczyk.

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Przetakiewicz, J., Nadolska-Orczyk, A., Kuć, D. et al. Tetraploid somatic hybrids of potato (Solanum tuberosum L.) obtained from diploid breeding lines. Cell Mol Biol Lett 12, 253–267 (2007). https://doi.org/10.2478/s11658-006-0068-5

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  • DOI: https://doi.org/10.2478/s11658-006-0068-5

Key words

  • Cytoplasmic hybrids
  • Heterokaryons
  • Molecular markers
  • Protoplast fusion