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Tetraploid somatic hybrids of potato (Solanum tuberosum L.) obtained from diploid breeding lines

Cellular & Molecular Biology Letters volume 12, pages 253–267 (2007)Cite this article

Abstract

Intraspecific somatic hybrids between 16 different diploid breeding lines of Solanum tuberosum L. were produced by PEG-induced fusion. Manually selected heterokaryons were cultured in a Millicells-CM using a post-fusion protoplast mixture. Plants were regenerated from calli derived from heterokaryons obtained from 10 out of 38 combinations of diploid lines. Of the tested putative somatic hybrids, 14.2% were diploid, 72.8% were tetraploid and 13% pentaploid. The DNA amplification pattern obtained with RAPD or semi-random primers confirmed that 6 fusion combinations were hybrids. In most cases, the morphological traits were intermediate to those of the diploid fusion partners. About 23.0% of the tested somatic hybrids showed variation in their morphology. Of the tested somatic hybrids, 78.0% flowered and 86.0% tuberized. The cytoplasm of 9 diploid lines and 6 somatic hybrid combinations was analysed. Two of the diploid lines had W/S chloroplasts and α or ε mitochondria; the remainder contained T chloroplasts and β mitochondria. All the analysed somatic hybrids carried T chloroplasts and β mitochondria.

Abbreviations

cpDNA:

chloroplast DNA

CTAB:

cetyltrimethyl ammonium bromide

FDA:

fluorescein diacetate

mtDNA:

mitochondrial DNA

NAA:

naphthaleneaceic acid

PEG:

polyethylene glicol

PVP:

polyvinylpyrrolidone

RAPD:

random amplification of polymorphic DNA

RFLP:

restriction fragments length polymorphism

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Authors and Affiliations

  1. Plant Transformation and Cell Engineering Department, Plant Breeding and Acclimatization Institute, Radzików, 05-870, Błonie, Poland

    Jarosław Przetakiewicz, Anna Nadolska-Orczyk, Dominik Kuć & Wacław Orczyk

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  1. Jarosław Przetakiewicz
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  2. Anna Nadolska-Orczyk
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  3. Dominik Kuć
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  4. Wacław Orczyk
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Correspondence to Wacław Orczyk.

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Przetakiewicz, J., Nadolska-Orczyk, A., Kuć, D. et al. Tetraploid somatic hybrids of potato (Solanum tuberosum L.) obtained from diploid breeding lines. Cell Mol Biol Lett 12, 253–267 (2007). https://doi.org/10.2478/s11658-006-0068-5

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  • DOI: https://doi.org/10.2478/s11658-006-0068-5

Key words

  • Cytoplasmic hybrids
  • Heterokaryons
  • Molecular markers
  • Protoplast fusion

Cellular & Molecular Biology Letters

ISSN: 1689-1392

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