- Short communication
Detection of the status of tumour-infiltrating CD4+ T-cell subpopulations in squamous cell carcinoma of the head and neck (HNSCC) using real-time RT-PCR detection of CD4 mRNA
Cellular & Molecular Biology Letters volume 12, pages 308–316 (2007)
Several studies have reported tumour infiltrating CD4+ T cells as a favourable prognostic factor in some types of cancer. We investigated 37 head and neck squamous cell carcinomas (HNSCC) at different stages, using immunohistochemical staining for CD4+ infiltrates and real-time reverse transcription polymerase chain reaction (RT-PCR) detection of CD4 mRNA. The CD4+ infiltrates were evaluated and expressed as a percentage according to the ratio of CD4+ T cells to epithelial cells in the cancer cell nests and to the overall inflammatory cell infiltrate in the tumor stroma. The CD4 mRNA expression level strongly correlated with the CD4+ infiltration score in the cancer epithelium (rs = 0.858, P < 0.001) and in the cancer stroma (rs = 0.797, P < 0.001). These results indicate that the real-time RT-PCR assay is a sensitive and reliable method for the detection of CD4 mRNA, and that it could be used to reassess CD4+ infiltration status in resected specimens from patients with HNSCC.
head and neck squamous cell carcinoma
major histocompatibility complex
non-fat dry milk
phosphate buffer saline
polymerase chain reaction
pathological Tumour Node Metastasis classification of cancer
reverse transcriptase polymerase chain reaction
sodium dodecyl sulfate
T-helper immune cells
- TRI reagent:
Statistical Package for the Social Sciences
International Union Against Cancer (Union Internationale Contre le Cancer)
Swain, S.L. T cell subsets and the recognition of MHC class. Immunol. Rev. 74 (1983) 129–142.
Nishimura, M.I., Avichezer, D., Custre, M.C., Lee, C.S., Chen, C., Parkhurst, M.R., Diamond, R.A., Robbins, P.F., Schwartzentruber, D.J. and Rosenberg, S.A. MHC class I-restricted recognition of a melanoma antigen by a human CD4+ tumour infiltrating lymphocyte. Canc. Res. 59 (1999) 6230–6238.
Veillette, A., Bookman, M.A., Horak, E.M. and Bolen, J.B. The CD4 and CD8 T-cell surface antigens are associated with the internal membrane tyrosine-protein kinase p56lck. Cell 55 (1988) 301–308.
Hung, K., Hayashi, R., Lafond-Walker, A., Lowenstein, C., Pardoll, D. and Levitsky, H. The central role of CD4+ T cells in antitumour immune response. J. Exp. Med. 188 (1998) 2357–2368.
Volpert, O.V., Fong, T., Koch, A.E., Peterson, J.D., Waltenbaugh, C., Tepper, R.I. and Bouck, N.P. Inhibition of angiogenesis by interleukin 4. J. Exp. Med. 188 (1998) 1039–1046.
Mattes, J., Hulett, M., Xie, W., Hogan, S., Rothenberg, M.E., Foster, P. and Parish, C. Immunotherapy of cytotoxic T cell-resistant tumours by T helper 2 cells: an eotaxin and STAT6-dependent process. J. Exp. Med. 197 (2003) 387–293.
Badoual, C., Hans, S., Rodriguez, J., Peyrard, S., Klein, C., Agueznay Nel, H., Mosseri, V., Laccourreye, O., Bruneval, P., Fridman, W.H., Brasnu, D.F. and Tartour, E. Prognostic value of tumour-infiltrating CD4+ T-cell subpopulations in head and neck cancers. Clin. Cancer Res. 15 (2006) 465–472.
Hiraoka, K., Miyamoto, M., Cho, Y., Suzuoki, M., Oshikiri, T., Nakakubo, Y., Itoh, T., Ohbuchi, T., Kondo, S. and Katoh, H. Concurrent infiltration by CD8+ T cells and CD4+ T cells is a favourable prognostic factor in nonsmall-cell lung carcinoma. Br. J. Cancer 94 (2) (2006) 275–280.
Macchetti, A.H., Marana, H.R., Silva, J.S., de Andrade, J.M., Ribeiro-Silva, A. and Bighetti, S. Tumour-infiltrating CD4+ T lymphocytes in early breast cancer reflect lymph node involvement. Clinics 61 (2006) 203–208.
Knutson, K.L, Disis, M.L. and Salazar, L.G. CD4 regulatory T cells in human cancer pathogenesis. Cancer Immunol. Immunother. (2006) in press.
Cho, Y., Miyamoto, M., Kato, K., Fukunaga, A., Shichinohe, T., Kawarada, Y., Hida, Y., Oshikiri, T., Kurokawa, T., Suzuoki, M., Nakakubo, Y., Hiraoka, K., Murakami, S., Shinohara, T., Itoh, T., Okushiba, S., Kondo, S. and Katoh, H. CD4+ and CD8+ T cells cooperate to improve prognosis of patients with esophageal squamous cell carcinoma. Cancer Res. 63 (2003) 1555–1559.
About this article
Cite this article
Smerkolj, S., Volavšek, M. & Glavač, D. Detection of the status of tumour-infiltrating CD4+ T-cell subpopulations in squamous cell carcinoma of the head and neck (HNSCC) using real-time RT-PCR detection of CD4 mRNA. Cell Mol Biol Lett 12, 308–316 (2007). https://doi.org/10.2478/s11658-007-0004-3