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The effect of growth medium on the antioxidant defense of Saccharomyces cerevisiae
Cellular & Molecular Biology Letters volume 12, pages 448–456 (2007)
We compared the oxidation of dihydrorhodamine 123, glutathione contents and activities of superoxide dismutase (SOD) and catalase for three wild-type strains of Saccharomyces cerevisiae grown on media with different carbon sources. The rate of oxidation of dihydrorhodamine 123 was much higher in respiring cells grown on ethanol or glycerol media than in fermenting cells grown on glucose medium. The total SOD activity was highest on glycerol medium and lowest on ethanol medium, while the catalase activity was highest on glycerol medium. The sequence of glutathione content values was: glucose > ethanol > glycerol.
- H2R 123:
reactive oxygen species
- YPD medium:
1% yeast extract, 1% Bacto-peptone, 2% glucose, YPG medium, 1% yeast extract, 1% Bacto-peptone,2% glycerol
- YPE medium:
1% yeast extract, 1% Bacto-peptone, 3% ethanol
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Macierzyńska, E., Grzelak, A. & Bartosz, G. The effect of growth medium on the antioxidant defense of Saccharomyces cerevisiae . Cell Mol Biol Lett 12, 448–456 (2007). https://doi.org/10.2478/s11658-007-0017-y
- Saccharomyces cerevisiae
- Reactive oxygen species
- Superoxide dismutase