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The effect of growth medium on the antioxidant defense of Saccharomyces cerevisiae

Abstract

We compared the oxidation of dihydrorhodamine 123, glutathione contents and activities of superoxide dismutase (SOD) and catalase for three wild-type strains of Saccharomyces cerevisiae grown on media with different carbon sources. The rate of oxidation of dihydrorhodamine 123 was much higher in respiring cells grown on ethanol or glycerol media than in fermenting cells grown on glucose medium. The total SOD activity was highest on glycerol medium and lowest on ethanol medium, while the catalase activity was highest on glycerol medium. The sequence of glutathione content values was: glucose > ethanol > glycerol.

Abbreviations

DTNB:

5,5′-dithiobis-(2-nitrobenzoic acid)

H2R 123:

dihydrorhodamine 123

MES:

2-morpholinoethanesulfonic acid

ROS:

reactive oxygen species

SOD:

superoxide dismutase

YPD medium:

1% yeast extract, 1% Bacto-peptone, 2% glucose, YPG medium, 1% yeast extract, 1% Bacto-peptone,2% glycerol

YPE medium:

1% yeast extract, 1% Bacto-peptone, 3% ethanol

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Correspondence to Ewa Macierzyńska.

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Macierzyńska, E., Grzelak, A. & Bartosz, G. The effect of growth medium on the antioxidant defense of Saccharomyces cerevisiae . Cell Mol Biol Lett 12, 448–456 (2007). https://doi.org/10.2478/s11658-007-0017-y

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  • DOI: https://doi.org/10.2478/s11658-007-0017-y

Key words

  • Yeast
  • Saccharomyces cerevisiae
  • Reactive oxygen species
  • Superoxide dismutase
  • Catalase
  • Glutathione