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Procaspase-9 is attached to the mitochondrial outer membrane in the early stages of apoptosis
Cellular & Molecular Biology Letters volume 12, pages 509–522 (2007)
Abstract
Procaspase-9 is the zymogen form of one of the apoptosis initiators, caspase-9. Its cellular location may differ depending on the cell type; it is found throughout the cytosol, although some of it may be associated with the mitochondria. Procaspase-9 relocates from the cytosol to the mitochondria shortly after the triggering of apoptosis in rat hepatocytes. We investigated whether the mitochondrial protein import machineries import procaspase-9. The combined results of protein import analyses, mitochondrial fractionation and protease treatments of intact and swollen mitochondria imply that procaspase-9 attaches to the outer surface of the mitochondrial outer membrane.
Abbreviations
- DHFR:
-
dihidrofolate-reductase
- MPP:
-
mitochondrial processing peptidase
- OXA:
-
oxidase assembly complex
- PK:
-
proteinase K
- PMSF:
-
phenylmethyl sulfonyl fluoride
- pSu9:
-
targeting sequence of subunit 9 of F0F1-ATPase
- SAM:
-
sorting and assembly machinery
- SDS-PAGE:
-
sodium dodecyl sulfate-polyacrylamide gel electrophoresis
- TIM:
-
translocase of the inner membrane
- TOB:
-
topogenesis of the outer membrane
- TOM:
-
translocase of the outer membrane
- Try:
-
trypsin
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Milisav, I., Šuput, D. Procaspase-9 is attached to the mitochondrial outer membrane in the early stages of apoptosis. Cell Mol Biol Lett 12, 509–522 (2007). https://doi.org/10.2478/s11658-007-0020-3
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DOI: https://doi.org/10.2478/s11658-007-0020-3