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Activation of the intrinsic and extrinsic pathways in high pressure-induced apoptosis of murine erythroleukemia cells
Cellular & Molecular Biology Letters volume 13, pages49–57(2008)
We previously demonstrated that caspase-3, an executioner of apoptosis, is activated in the pressure-induced apoptosis of murine erythroleukemia (MEL) cells (at 100 MPa). Here, we examined the pathway of caspase-3 activation using peptide substrates and caspase inhibitors. Using the substrates of caspases-8 and -9, it was found that both are activated in cells under high pressure. The production of nuclei with sub-G1 DNA content in 100 MPa-treated MEL cells was suppressed by inhibitors of caspases-8 and -9, and pan-caspase. In 100 MPa-treated cells, pan-caspase inhibitor partially prevented the cytochrome c release from the mitochondria and the breakdown of mitochondrial membrane potential. These results suggest that the intrinsic and extrinsic pathways are activated in apoptotic signaling during the high pressure-induced death of MEL cells.
- RNase A:
reactive oxygen species
Okada, H. and Mak, T.W. Pathways of apoptotic and non-apoptotic death in tumour cells. Nature Rev. Cancer 4 (2004) 592–603.
Berghe, T.V., van Loo, G., Saelens, X., van Gurp, M., Brouckaert, G., Kalai, M., Declercq, W. and Vandenabeele, P. Differential signaling to apoptotic and necrotic cell death by Fas-associated death domain protein FADD. J. Biol. Chem. 279 (2004) 7925–7933.
Majno, G. and Joris, I. Apoptosis, oncosis, and necrosis: an overview of cell death. Am. J. Pathol. 146 (1995) 3–15.
Green, D.R. Apoptotic pathways: paper wraps stone blunts scissors. Cell 102 (2000) 1–4.
Scoltock, A.B. and Cidlowski, J.A. Activation of intrinsic and extrinsic pathways in apoptotic signaling during UV-C-induced death of Jurkat cells: the role of caspase inhibition. Exp. Cell Res. 297 (2004) 212–223.
Rudner, J., Lepple-Wienhues, A., Budach, W., Berschauer, J., Friedrich, B., Wesselborg, S., Schulze-Osthoff, K. and Belka, C. Wild-type, mitochondrial and ER-restricted Bcl-2 inhibit DNA damage-induced apoptosis but do not affect death receptor-induced apoptosis. J. Cell Sci. 114 (2001) 4164–4172.
Matsuzawa, A. and Ichijo, H. Molecular mechanisms of the decision between life and death: regulation of apoptosis by apoptosis signal-regulating kinase 1. J. Biochem. 130 (2001) 1–8.
Schuler, M., Bossy-Wetzel, E., Goldstein, J.C., Fitzgerald, P. and Green, D.R. p53 induces apoptosis by caspase activation through mitochondrial cytochrome c release. J. Biol. Chem. 275 (2000) 7337–7342
Rosse, T., Olivier, R., Monney, L., Rager, M., Conus, S., Fellay, I., Jansen, B. and Borner, C. Bcl-2 prolongs cell survival after Bax-induced release of cytochrome c. Nature 391 (1998) 496–499.
Adams, J. M. and Cory, S. Life-or-death decisions by the Bcl-2 protein family. Trends Biochem. Sci. 26 (2001) 61–66.
Take, J., Yamaguchi, T., Mine, N. and Terada, S. Caspase activation in high-pressure-induced apoptosis of murine erythroleukemia cells. Jpn. J. Physiol. 51 (2001) 193–199.
Takano, K.J., Takano, T., Yamanouchi, Y. and Satou, T. Pressure-induced apoptosis in human lymphoblasts. Exp. Cell Res. 235 (1997)155–160.
Matsumoto, M., Yamaguchi, T., Fukumaki, Y., Yasunaga, R. and Terada, S. High pressure induces G2 arrest in murine erythroleukemia cells. J. Biochem. 123 (1998) 87–93.
Luo, X., Budihardjo, I., Zou, H., Slaughter, C. and Wang, X. Bid, a Bcl2 interacting protein, mediates cytochrome c release from mitochondria in response to activation of cell surface death receptors. Cell 94 (1998) 481–490.
Sohn, D., Schulze-Osthoff, K. and Janicke, R.U. Caspase-8 can be activated by interchain proteolysis without receptor-triggered dimerization during drug-induced apoptosis. J. Biol. Chem. 280 (2005) 5267–5273.
Enari, M., Sakahira, H., Yokoyama, H., Okawa, K., Iwamatsu, A. and Nagata, S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 391 (1998) 43–50.
Hassepass, I., Voit, R. and Hoffmann, I. Phosphorylation at serine 75 is required for UV-mediated degradation of human Cdc 25A phosphatase at the S-phase checkpoint. J. Biol. Chem. 278 (2003) 29824–29829.
Lee, J-H. and Paull, T.T. Direct activation of the ATM protein kinase by the Mre11/Rad 50/Nbs1 complex. Science 304 (2004) 93–96.
Ward, I. M., Minn, K. and Chen J. UV-induced ataxia-telangiectasia-mutated and Rad3-related (ATR) activation requires replication stress. J. Biol. Chem. 279 (2004) 9677–9680.
Takahashi, H., Yamaguchi, T., Koga, M., Kageura, H. and Terada, S. DNA replication reaction in Xenopus cell-free system is suppressed by high pressure. Cell. Mol. Biol. Lett. 9 (2004) 423–427.
Weber, G. and Drickamer, H.G. The effect of high pressure upon proteins and other biomolecules. Q. Rev. Biophys. 16 (1983) 89–112.
Aertsen, A., DeSpiegeleer, P, Vanoirbeek, K., Lavilla, M. and Michiels, C.W. Induction of oxidative stress by high hydrostatic pressure in Escherichia coli. Appl. Environ. Microbiol. 71 (2005) 2226–2231.
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Yamaguchi, T., Hashiguchi, K., Katsuki, S. et al. Activation of the intrinsic and extrinsic pathways in high pressure-induced apoptosis of murine erythroleukemia cells. Cell Mol Biol Lett 13, 49–57 (2008). https://doi.org/10.2478/s11658-007-0034-x
- Cytochrome c
- Flow cytometry
- Membrane potential
- High pressure