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Platelet-activating factor changes in phospholipid extracts from the plasma, peripheral blood mononuclear cells and bone marrow mononuclear cells of patients with acute leukemia — A 31P MRS in vitro study

Abstract

The aim of this investigation was to evaluate the changes in PAF concentrations in the plasma, PBMC and BMMC of patients with acute lymphoblastic leukemia (ALL) and acute myeloblastic leukemia (AML). The plasma was from 23 healthy volunteers (HV) and 44 patients with AL (16 ALL, 28 AML). The PBMC were from 15 HV and 55 patients with AL (18 ALL, 37 AML), and the BMMC from 40 patients with AL (11 ALL, 29 AML). Methanol-chloroform phospholipid extraction from 60 × 106 cells (PBMC or BMMC) was performed according to a modified version of Folch’s method. 31P MRS data was obtained on an AMX 300 Bruker spectrometer (7.05 T). The PAF concentration in the plasma of the patients with ALL or AML was lower than that for the healthy volunteers. The PAF concentration in the plasma of the patients with ALL did not differ significantly from that of the patients with AML. In the case of both the PBMC and BMMC, the PAF concentration was significantly diminished in patients with ALL relative to the concentration for those with AML and for the healthy volunteers. No differences were observed in the PAF concentrations for the AML patients and the healthy volunteers.

Abbreviations

AL:

acute leukemia

ALL:

acute lymphoblastic leukemia

AML:

acute myeloblastic leukemia

BMMC:

bone marrow mononuclear cells

MRS:

magnetic resonance spectroscopy

PAF (CPLAS):

platelet-activating factor, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine

PBMC:

peripheral blood mononuclear cells

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Correspondence to Małgorzata Kuliszkiewicz-Janus.

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Kuliszkiewicz-Janus, M., Tuz, M.A., Kiełbiński, M. et al. Platelet-activating factor changes in phospholipid extracts from the plasma, peripheral blood mononuclear cells and bone marrow mononuclear cells of patients with acute leukemia — A 31P MRS in vitro study. Cell Mol Biol Lett 13, 58–66 (2008). https://doi.org/10.2478/s11658-007-0035-9

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Key words

  • PAF
  • Acute leukemia
  • 31P MRS in vitro