Skip to main content

The effect of tributyltin on human eosinophylic leukemia EoL-1 cells

Abstract

Organotin compounds are chemicals that are widely used in industry and agriculture as plastic stabilizers, catalysts and biocides. Many of them, including tributyltin (TBT), have been detected in human food and, as a consequence, detectable levels have been found in human blood. As organotin compounds were shown to possess immunotoxic activity, we focused our attention on the effect of TBT on the basic determinants of the function of eosinophils, i.e. cell adhesiveness and motility. We used human eosinophylic leukemia EoL-1 cells, a common in vitro cellular model of human eosinophils. Here, we demonstrate that TBT causes a dose-dependent decrease in the viability of EoL-1 cells. When administered at sub-lethal concentrations, TBT significantly decreases the adhesion of EoL-1 cells to human fibroblasts (HSFs) and inhibits their migration on fibroblast surfaces. Since the basic function of eosinophils is to invade inflamed tissues, our results indicate that TBT, and possibly other organotin compounds, may affect major cellular properties involved in the determination of in vivo eosinophil function.

Abbreviations

HSFs:

human skin fibroblasts

SEM:

standard error of the mean

TBT:

tributyltin

References

  1. 1.

    Whalen, M.M., Logananthan, B.G. and Kannan, K. Immunotoxicity of environmentally relevant concentrations of butyltins on human natural killer cells in vitro. Env. Res. 81 (1999) 108–116.

    Article  CAS  Google Scholar 

  2. 2.

    Fent, K. Ecotoxicology of organotin compounds. Crit. Rev. Toxicol. 26 (1996) 1–117.

    PubMed  Article  CAS  Google Scholar 

  3. 3.

    Hoch, M. Organotin compounds in the environment. Appl. Geochem. 16 (2001) 719–743.

    Article  CAS  Google Scholar 

  4. 4.

    Appel, K.E. Organotin compounds: Toxicokinetic aspects. Drug. Met. Rev. 36 (2004) 763–786.

    Article  CAS  Google Scholar 

  5. 5.

    Silberstein D.A. Eosinophil function in health and disease. Crit. Rev. Oncol. Hematol. 19 (1995) 47–77.

    PubMed  Article  CAS  Google Scholar 

  6. 6.

    Mayumi, M. EoL-1, a human eosinophilic cell line. Leuk. Lymphoma 7 (1992) 243–250.

    PubMed  Article  CAS  Google Scholar 

  7. 7.

    Wong, C.K., Ho, C.Y., Lam, C.W., Zhang, J.P. and Hjelm, N.M. Differentiation of a human eosinophilic leukemic cell line, EoL-1: characterization by the expression of cytokine receptors, adhesion molecules, CD95 and eosinophilic cationic protein (ECP). Immunol. Lett. 68 (1999) 317–323.

    PubMed  Article  CAS  Google Scholar 

  8. 8.

    Szydłowska, H., Zaporowska, E., Kuszlik-Jochym, K., Korohoda, W. and Branny, J. Membranolytic activity of detergents as studied with cell viability tests. Folia Histochem. Cytochem. 16 (1978) 69–78.

    Google Scholar 

  9. 9.

    Wójciak, B. and Korohoda, W. Ehrlich ascites tumour cells show tissuespecific adherence and modify their shape upon contact with embryonic fibroblasts and myotubes. J. Cell Sci. 97 (1990) 433–438.

    PubMed  Google Scholar 

  10. 10.

    Korohoda, W., Golda, J., Sroka, J., Wojnarowicz, A., Jochym, P. and Madeja, Z. Chemotaxis of Amoeba proteus in the developing pH gradient within a pocket-like chamber studied with the computer assisted method. Cell Motil. Cytoskel. 38 (1997) 38–53.

    Article  CAS  Google Scholar 

  11. 11.

    Sroka, J., Kamiński, R., Michalik, M., Madeja, M., Przestalski, S. and Korohoda, W. The effect of triethyllead on the motile activity of Walker 256 carcinosarcoma cells. Cell. Mol. Biol. Lett. 9 (2004) 15–30.

    PubMed  CAS  Google Scholar 

  12. 12.

    Miękus, K. and Madeja, Z. Genistein inhibits the contact-stimulated migration of prostate cancer cells. Cell. Mol. Biol. Lett. 12 (2007) 348–361

    Article  CAS  Google Scholar 

  13. 13.

    Reader, S., Moutardier, V. and Denizeau, F. Tributyltin triggers apoptosis in trout hepatocytes: the role of Ca2+, protein kinase C and proteases. Biochim. Biophys. Acta. 1448 (1999) 473–485.

    PubMed  Article  CAS  Google Scholar 

  14. 14.

    Umebayashi, C., Oyama, Y., Chikahisa-Muramastu, L., Nakao, H., Nishizaki, Y., Nakata, M., Soeda, F., Takahama, K., Seibert, H., Morchel, S. and Gulden, M. Tri-n-butyltin-induced cytotoxicity on rat thymocytes in presence and absence of serum. Toxicol. In Vitro 18 (2004) 55–61.

    PubMed  Article  CAS  Google Scholar 

  15. 15.

    Zucker, R.M., Elstein, K.H., Easterling, R.E. and Massaro, E.J. Flow cytometric comparison of the effects of trialkyltins on the murine erythroleukemic cell. Toxicology 58 (1989) 107–119.

    PubMed  Article  CAS  Google Scholar 

  16. 16.

    Seibert, H., Morchel, S. and Gulden, M. Cytotoxic potency of trialkyltins to C6 glioma cells in vitro: impact of exposure conditions. Cell. Biol. Toxicol. 20 (2004) 273–283.

    PubMed  Article  CAS  Google Scholar 

  17. 17.

    Owen, W.F. Jr, Rothenberg, M.E., Silberstein, D.S., Gasson, J.C., Stevens, R.L., Austen, K.F. and Soberman, R.J. Regulation of human eosinophil viability, density, and function by granulocyte/macrophage colonystimulating factor in the presence of 3T3 fibroblasts. J. Exp. Med. 166 (1987) 129–141.

    PubMed  Article  CAS  Google Scholar 

  18. 18.

    Rothenberg, M.E., Owen, W.F. Jr, Silberstein, D.S., Woods, J., Soberman, R.J., Austen, K.F. and Stevens, R.L. Human eosinophils have prolonged survival, enhanced functional properties, and become hypodense when exposed to human interleukin 3. J. Clin. Invest. 81 (1988) 1986–1992.

    PubMed  CAS  Article  Google Scholar 

  19. 19.

    Solomon, A., Shmilowich, R., Shasha, D., Frucht-Pery, J., Pe’er, J., Bonini, S. and Levi-Schaffer, F. Conjunctival fibroblasts enhance the survival and functional activity of peripheral blood eosinophils in vitro. Invest. Ophthalmol. Vis. Sci. 41 (2000) 1038–1044.

    PubMed  CAS  Google Scholar 

  20. 20.

    Gomes, I., Mathur, S.K., Espenshade, B.M., Mori, Y., Varga, J. and Ackerman, S.J. Eosinophil-fibroblast interactions induce fibroblast IL-6 secretion and extracellular matrix gene expression: implications in fibrogenesis. J. Allergy Clin. Immunol. 116 (2005) 796–804.

    PubMed  Article  CAS  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Jolanta Sroka.

Additional information

Paper authored by participants of the international conference: XXXIV Winter School of the Faculty of Biochemistry, Biophysics and Biotechnology of Jagiellonian University, Zakopane, March 7–11, 2007, “The Cell and Its Environment”. Publication costs were covered by the organisers of this meeting.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Sroka, J., Włosiak, P., Wilk, A. et al. The effect of tributyltin on human eosinophylic leukemia EoL-1 cells. Cell Mol Biol Lett 13, 67–73 (2008). https://doi.org/10.2478/s11658-007-0037-7

Download citation

Key words

  • Tributyltin
  • Cytotoxicity
  • Cell adhesion
  • Cell migration
  • EoL-1 cells