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The effect of tributyltin on human eosinophylic leukemia EoL-1 cells

Cellular & Molecular Biology Letters volume 13, pages 67–73 (2008)Cite this article

Abstract

Organotin compounds are chemicals that are widely used in industry and agriculture as plastic stabilizers, catalysts and biocides. Many of them, including tributyltin (TBT), have been detected in human food and, as a consequence, detectable levels have been found in human blood. As organotin compounds were shown to possess immunotoxic activity, we focused our attention on the effect of TBT on the basic determinants of the function of eosinophils, i.e. cell adhesiveness and motility. We used human eosinophylic leukemia EoL-1 cells, a common in vitro cellular model of human eosinophils. Here, we demonstrate that TBT causes a dose-dependent decrease in the viability of EoL-1 cells. When administered at sub-lethal concentrations, TBT significantly decreases the adhesion of EoL-1 cells to human fibroblasts (HSFs) and inhibits their migration on fibroblast surfaces. Since the basic function of eosinophils is to invade inflamed tissues, our results indicate that TBT, and possibly other organotin compounds, may affect major cellular properties involved in the determination of in vivo eosinophil function.

Abbreviations

HSFs:

human skin fibroblasts

SEM:

standard error of the mean

TBT:

tributyltin

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Authors and Affiliations

  1. Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, ul. Gronostajowa 7, 30-378, Kraków, Poland

    Jolanta Sroka, Przemysław Włosiak, Anna Wilk, Justyna Antonik, Jarosław Czyż & Zbigniew Madeja

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  1. Jolanta Sroka
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  2. Przemysław Włosiak
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  3. Anna Wilk
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  4. Justyna Antonik
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  5. Jarosław Czyż
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  6. Zbigniew Madeja
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Correspondence to Jolanta Sroka.

Additional information

Paper authored by participants of the international conference: XXXIV Winter School of the Faculty of Biochemistry, Biophysics and Biotechnology of Jagiellonian University, Zakopane, March 7–11, 2007, “The Cell and Its Environment”. Publication costs were covered by the organisers of this meeting.

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Sroka, J., Włosiak, P., Wilk, A. et al. The effect of tributyltin on human eosinophylic leukemia EoL-1 cells. Cell Mol Biol Lett 13, 67–73 (2008). https://doi.org/10.2478/s11658-007-0037-7

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  • DOI: https://doi.org/10.2478/s11658-007-0037-7

Key words

  • Tributyltin
  • Cytotoxicity
  • Cell adhesion
  • Cell migration
  • EoL-1 cells

Cellular & Molecular Biology Letters

ISSN: 1689-1392

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