- Research Article
The construction and characteristics of a BAC library for Cucumis sativus L. ‘B10’
Cellular & Molecular Biology Letters volume 13, pages 74–91 (2008)
Cloning using bacterial artificial chromosomes (BACs) can yield high quality genomic libraries, which are used for the physical mapping, identification and isolation of genes, and for gene sequencing. A BAC genomic library was constructed from high molecular weight DNA (HMW DNA) obtained from nuclei of the cucumber (Cucumis sativus L. cv. Borszczagowski; B10 line). The DNA was digested with the HindIII restriction enzyme and ligated into the pCC1BAC vector. The library consists of 34,560 BAC clones with an average insert size of 135 kb, and 12.7x genome coverage. Screening the library for chloroplast and mitochondrial DNA content indicated an exceptionally low 0.26% contamination with chloroplast DNA and 0.3% with mitochondrial DNA.
bacterial artificial chromosome
albumin from bovine serum
- HMW DNA:
high molecular weight DNA
low melting agarose
polymerase chain reaction
pulsed field gel electrophoresis
sequence-characterised amplified regions
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Gutman, W., Pawełkowicz, M., Woycicki, R. et al. The construction and characteristics of a BAC library for Cucumis sativus L. ‘B10’. Cell Mol Biol Lett 13, 74–91 (2008). https://doi.org/10.2478/s11658-007-0038-6
- Cucumis sativus L.
- Bacterial artificial chromosome
- High molecular weight DNA
- Pulsed field gel electrophoresis