Open Access

The transcription reinitiation properties of RNA polymerase III in the absence of transcription factors

Cellular & Molecular Biology LettersAn International Journal200713:41

https://doi.org/10.2478/s11658-007-0041-y

Received: 30 May 2007

Accepted: 31 July 2007

Published: 29 October 2007

Abstract

Transcription reinitiation by RNA polymerase (Pol) III proceeds through facilitated recycling, a process by which the terminating Pol III, assisted by the transcription factors TFIIIB and TFIIIC, rapidly reloads onto the same transcription unit. To get further insight into the Pol III transcription mechanism, we analyzed the kinetics of transcription initiation and reinitiation of a simplified in vitro transcription system consisting only of Pol III and template DNA. The data indicates that, in the absence of transcription factors, first-round transcription initiation by Pol III proceeds at a normal rate, while facilitated reinitiation during subsequent cycles is compromised.

Key words

RNA polymerase III Transcription reinitiation TFIIIB G-less cassette

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