- Short Communication
hnRNP-R regulates the PMA-induced c-fos expression in retinal cells
Cellular & Molecular Biology Letters volume 13, pages 303–311 (2008)
This study focused on the function of hnRNP-R in the regulation of c-fos expression. We demonstrated that hnRNP-R accelerated the rise and decline phases of c-fos mRNAs and Fos proteins, allowing PMA to induce an augmented pulse response of c-fos expression. Then, we examined the role of the c-fos-derived AU-rich element (ARE) in hnRNP-R-regulated mRNA degradation. Studies with the ARE-GFP reporter gene showed that hnRNP-R significantly reduced the expression of GFP with an inserted ARE. Moreover, immunoprecipitation-RT-PCR analysis demonstrated that in R28 cells and rat retinal tissues, the c-fos mRNA was co-immunoprecipitated with hnRNP-R. These findings indicate that hnRNP-R regulates the c-fos expression in retinal cells, and that the ARE of c-fos mRNAs contributes to this regulation.
green fluorescence protein
phorbol 12-myristate 13-acetate
spinal muscular atrophy
Sheng, M. and Greenberg, M.E. The regulation and function of c-fos and other immediate early genes in the nervous system. Neuron 4 (1990) 477–485.
Kovacs, K.J. c-fos as a transcription factor: a stressful (re)view from a functional map. Neurochem. Int. 33 (1998) 287–297.
Rahmsdorf, H.J., Schonthal, A., Angel, P., Litfin, M., Ruther, U. and Herrlich, P. Posttranscriptional regulation of c-fos mRNA expression. Nucleic Acids Res. 15 (1987) 1643–1659.
Sariban, E., Luebbers, R. and Kufe, D. Transcriptional and posttranscriptional control of c-fos gene expression in human monocytes. Mol. Cell Biol. 8 (1988) 340–346.
Mitchell, P. and Tollervey, D. mRNA turnover. Curr. Opin. Cell Biol. 13 (2001) 320–325.
Grosset, C., Chen, C.Y., Xu, N., Sonenberg, N., Jacquemin-Sablon, H. and Shyu, A.B. A mechanism for translationally coupled mRNA turnover: interaction between the poly(A) tail and a c-fos RNA coding determinant via a protein complex. Cell 103 (2000) 29–40.
Chen, C.Y. and Shyu, A.B. Au-Rich Elements-Characterization and importance in messenger-RNA degradation. Trends Biochem. Sci. 20 (1995) 465–470.
Krecic, A.M. and Swanson, M.S. hnRNP complexes: composition, structure, and function. Curr. Opin. Cell Biol. 11 (1999) 363–371.
Mourelatos, Z., Abel, L., Yong, J., Kataoka, N. and Dreyfuss, G.. SMN interacts with a novel family of hnRNP and spliceosomal proteins. EMBO J. 20 (2001) 5443–5452.
Rossoll, W., Kroning, A.K., Ohndorf, U.M., Steegborn, C., Jablonka, S. and Sendtner, M. Specific interaction of Smn, the spinal muscular atrophy determining gene product, with hnRNP-R and gry-rbp/hnRNP-Q: a role for Smn in RNA processing in motor axons? Hum. Mol. Genet. 11 (2002) 93–105.
Rossoll, W., Jablonka, S., Andreassi, C., Kroning, A.K., Karle, K., Monani, U.R. and Sendtner, M. Smn, the spinal muscular atrophy-determining gene product, modulates axon growth and localization of beta-actin mRNA in growth cones of motoneurons. J. Cell Biol. 163 (2003) 801–812.
Kim, T.D., Kim, J.S., Kim, J.H., Myung, J., Chae, H.D., Woo, K.C., Jang, S.K., Koh, D.S and Kim, K.T. Rhythmic serotonin N-acetyltransferase mRNA degradation is essential for the maintenance of its circadian oscillation. Mol. Cell Biol. 25 (2005) 3232–3246.
Huang, J., Chen, X.H., Wu, K. and Xu, P. Cloning and expression of a novel isoform of heterogeneous nuclear ribonucleoprotein-R. Neuroreport 16 (2005) 727–730.
Esnault, S. and Malter, J.S. Hyaluronic acid or TNF-alpha plus fibronectin triggers granulocyte macrophage-colony-stimulating factor mRNA stabilization in eosinophils yet engages differential intracellular pathways and mRNA binding proteins. J. Immunol. 171 (2003) 6780–6787.
Chen, X., Guo, L., Lin, W. and Xu, P. Expression of Tra2beta isoforms is developmentally regulated in a tissue-and temporal-specific pattern. Cell Biol. Int. 27 (2003) 491–496.
Amemiya, T., Kambe, T., Fukumori, R. and Kubo, T. Role of protein kinase C beta in phorbol ester-induced c-fos gene expression in neurons of normotensive and spontaneously hypertensive rat brains. Brain Res. 1040 (2005) 129–136.
Humphries, A. and Carter, D.A. Circadian dependency of nocturnal immediate-early protein induction in rat retina. Biochem. Biophys. Res. Commun. 320 (2004) 551–556.
Schiavi, S.C., Wellington, C.L., Shyu, A.B., Chen, C.Y., Greenberg, M.E. and Belasco, J.G. Multiple elements in the c-fos protein-coding region facilitate mRNA deadenylation and decay by a mechanism coupled to translation. J. Biol. Chem. 269 (1994) 3441–3448.
Panchision, D.M., Gerwin, C.M., DeLorenzo, R.J. and Jakoi, E.R. Glutamate receptor activation regulates mRNA at both transcriptional and posttranscriptional levels. J. Neurochem. 65 (1995) 969–977.
About this article
Cite this article
Huang, J., Li, SJ., Chen, XH. et al. hnRNP-R regulates the PMA-induced c-fos expression in retinal cells. Cell Mol Biol Lett 13, 303–311 (2008). https://doi.org/10.2478/s11658-008-0002-0
- mRNA turnover