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hnRNP-R regulates the PMA-induced c-fos expression in retinal cells

Abstract

This study focused on the function of hnRNP-R in the regulation of c-fos expression. We demonstrated that hnRNP-R accelerated the rise and decline phases of c-fos mRNAs and Fos proteins, allowing PMA to induce an augmented pulse response of c-fos expression. Then, we examined the role of the c-fos-derived AU-rich element (ARE) in hnRNP-R-regulated mRNA degradation. Studies with the ARE-GFP reporter gene showed that hnRNP-R significantly reduced the expression of GFP with an inserted ARE. Moreover, immunoprecipitation-RT-PCR analysis demonstrated that in R28 cells and rat retinal tissues, the c-fos mRNA was co-immunoprecipitated with hnRNP-R. These findings indicate that hnRNP-R regulates the c-fos expression in retinal cells, and that the ARE of c-fos mRNAs contributes to this regulation.

Abbreviations

ARE:

AU-rich element

DMSO:

dimethyl sulfoxide

GFP:

green fluorescence protein

PMA:

phorbol 12-myristate 13-acetate

SMA:

spinal muscular atrophy

UTR:

3′-untranslated region

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Correspondence to Xian-Hua Chen.

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Huang, J., Li, SJ., Chen, XH. et al. hnRNP-R regulates the PMA-induced c-fos expression in retinal cells. Cell Mol Biol Lett 13, 303–311 (2008). https://doi.org/10.2478/s11658-008-0002-0

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  • DOI: https://doi.org/10.2478/s11658-008-0002-0

Keywords

  • hnRNP-R
  • Retina
  • c-fos
  • mRNA turnover
  • ARE