- Research Article
The cell type-specific effect of TAp73 isoforms on the cell cycle and apoptosis
Cellular & Molecular Biology Letters volume 13, pages 404–420 (2008)
p73, a member of the p53 family, exhibits activities similar to those of p53, including the ability to induce growth arrest and apoptosis. p73 influences chemotherapeutic responses in human cancer patients, in association with p53. Alternative splicing of the TP73 gene produces many p73 C- and N-terminal isoforms, which vary in their transcriptional activity towards p53-responsive promoters. In this paper, we show that the C-terminal spliced isoforms of the p73 protein differ in their DNA-binding capacity, but this is not an accurate predictor of transcriptional activity. In different p53-null cell lines, p73β induces either mitochondrial-associated or death receptor-mediated apoptosis, and these differences are reflected in different gene expression profiles. In addition, p73 induces cell cycle arrest and p21WAF1 expression in H1299 cells, but not in Saos-2. This data shows that TAp73 isoforms act differently depending on the tumour cell background, and have important implications for p73-mediated therapeutic responses in individual human cancer patients.
TRAF and TNF receptor-associated protein-22
apurinic/apyrimidinic endonuclease 1/redox factor
apoptosis stimulating proteins of p53
cyclin-dependent protein kinase-1
Dulbecco's modified Eagle medium
trail death receptor
electrophoretic mobility-shift assay
foetal bovine serum
in vitro transcription/translation
microtubule-associated protein 4
p53-induced protein with a death domain
promyelocytic leukaemia gene
SDS-polyacrylamide gel electrophoresis
TNF receptor-associated factor
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These authors contributed equally to this paper
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Holcakova, J., Ceskova, P., Hrstka, R. et al. The cell type-specific effect of TAp73 isoforms on the cell cycle and apoptosis. Cell Mol Biol Lett 13, 404–420 (2008). https://doi.org/10.2478/s11658-008-0011-z
- DNA binding
- Cell cycle