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The influence of LTS-4, a saponoside from Lysimachia thyrsiflora L., on human skin fibroblasts and human melanoma cells

Abstract

We investigated the effect of a triterpene saponoside from Lysimachia thyrsiflora L. upon the viability, proliferation, morphology and cell motility of human melanoma HTB-140 cells and human skin fibroblasts (HSFs). The compound, denoted LTS-4, decreased the viability and rate of cell growth of both cell types in a time-and dose-dependent manner, and proved cytotoxic against cancer cells at significantly lower concentrations than for fibroblasts. LTS-4 also affected the morphology of the examined cells, causing vacuolisation and actin cytoskeleton disintegration, and had an inhibitory effect on the tumour cell motility.

Abbreviations

CME:

coefficient of movement efficiency

PBS:

phosphate buffered saline

TLC:

thin-layer chromatography

TRITC phalloidin:

phalloidin-conjugated tetramethylrhodamine B isothiocyanate

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Correspondence to Agnieszka Galanty.

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Galanty, A., Michalik, M., Sędek, Ł. et al. The influence of LTS-4, a saponoside from Lysimachia thyrsiflora L., on human skin fibroblasts and human melanoma cells. Cell Mol Biol Lett 13, 585–598 (2008). https://doi.org/10.2478/s11658-008-0013-x

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  • DOI: https://doi.org/10.2478/s11658-008-0013-x

Key words

  • Triterpene saponoside
  • Cytotoxicity
  • Lysimachia thyrsiflora L.
  • Melanoma
  • Fibroblasts