- Short Communication
The mitochondrial localization of RelB and NFATx in immature T cells
Cellular & Molecular Biology Letters volume 13, pages 493–501 (2008)
In order to exert their activity, transcription factors must be transported to the nucleus. Certain transcription factors have also been found on mitochondria. Here, the localization of RelB and NFATx in the mitochondrial fractions of normal thymocytes and thymic lymphoma cells is shown for the first time. CREB was only found in the nucleus, while p50 (NFκB) was found in both the nucleus and the cytoplasm, but outside the mitochondria. The translocation of transcription factors to the mitochondria is differentially regulated. Unlike RelB, which is always present in the mitochondrial fraction, NFATx appeared on the mitochondria in cells treated with ionomycin together with an immunosuppressant and inhibitor of calcineurin (FK506). This data reveals that the mitochondrial localization of some transcription factors is precisely controlled by a calcium signal sensitive to FK506 in T cells.
activator protein 1
cAMP response element-binding protein
foetal calf serum
60-kDA heat-shock protein
c-Jun N-terminal kinase
- NFATx (NFATc3):
nuclear factor of activated T cells 3
nuclear factor κB
mitogenactivated protein kinase p38
sodium dodecyl sulphate
SDS-polyacrylamide gel electrophoresis
Tris buffer solution
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Stasik, I., Rapak, A., Zioło, E. et al. The mitochondrial localization of RelB and NFATx in immature T cells. Cell Mol Biol Lett 13, 493–501 (2008). https://doi.org/10.2478/s11658-008-0019-4
- Mitochondrial translocation
- Transcription factors
- Thymic lymphoma