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The GTPase domain of Galphao contributes to the functional interaction of Galphao with the promyelocytic leukemia zinc finger protein

Abstract

Go, one of the most abundant heterotrimeric G proteins in the brain, is classified as a member of the Gi/Go family based on its homology to Gi proteins. Recently, we identified promyelocytic leukemia zinc finger protein (PLZF) as a candidate downstream effector for the alpha subunit of Go (Gαo). Activated Gαo interacts with PLZF and augments its function as a repressor of transcription and cell growth. G protein-coupled receptor-mediated Gαo activation also enhanced PLZF function. In this study, we determined that the GTPase domain of Gαo contributes to Gαo:PLZF interaction. We also showed that the Gαo GTPase domain is important in modulating the function of PLZF. This data indicates that the GTPase domain of Gαo may be necessary for the functional interaction of Gαo with PLZF.

Abbreviations

β-gal:

β-galactosidase

BrdU:

bromodeoxyuridine

Gαo:

the alpha subunit of Go

G proteins:

heterotrimeric GTP-binding proteins

GST:

glutathione-S-transferase

PKA:

cAMP-dependent protein kinase

PLZF:

promyelocytic leukemia zinc finger protein

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Correspondence to Sung Ho Ghil.

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Key words

  • Cell growth
  • Differentiation
  • Domain
  • G protein