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A consensus map of chromosome 6R in rye (Secale cereale L.)

Abstract

Four F2 mapping populations derived from crosses between rye inbred lines DS2×RXL10, 541×Ot1-3, S120×S76 and 544×Ot0-20 were used to develop a consensus map of chromosome 6R. Thirteen marker loci that were polymorphic in more than one mapping population constituted the basis for the alignment of the four maps using the JoinMap v. 3.0 software package. The consensus map consists of 104 molecular marker loci including RFLPs, RAPDs, AFLPs, SSRs, ISSRs, SCARs, STSs and isozymes. The average distance between the marker loci is 1.3 cM, and the total map length is 135.5 cM. This consensus map may be used as a source of molecular markers for the rapid development of new maps of chromosome 6R in any mapping population.

Abbreviations

AFLP:

amplified fragment length polymorphism

cM:

centimorgan

ISSR:

inter-simple sequence repeat

LOD:

logarithm of odds

QTL:

quantitative trait locus

RAPD:

random amplified polymorphic DNA

RFLP:

restriction fragment length polymorphism

SCAR:

sequence-characterized amplified region

SSR:

simple sequence repeat

STS:

sequence-tagged site

References

  1. Geiger, H.H. Strategies of hybrid rye breeding. Vortr. Pflanzenzüchtg. 71 (2007) 1–5.

    Google Scholar 

  2. Devos, K.M., Atkinson, M.D., Chinoy, C.N., Francis, H.A., Harcourt, R.L., Koebner, R.M.D., Liu, C.J., Masojć, P., Xie, D.X. and Gale, M.D. Chromosomal rearrangements in the rye genome relative to that of wheat. Theor. Appl. Genet. 85 (1993) 673–680.

    Article  CAS  Google Scholar 

  3. Philipp, U., Wehling, P. and Wricke, G. A linkage map of rye. Theor. Appl. Genet. 88 (1994) 243–248.

    Article  CAS  Google Scholar 

  4. Loarce, Y., Hueros, G. and Ferrer, E. A molecular linkage map of rye. Theor. Appl. Genet. 93 (1996) 1112–1118.

    Article  CAS  Google Scholar 

  5. Korzun, V., Malyshev, S., Kartel, N., Westermann, T., Weber, W.E. and Börner, A. A genetic linkage map of rye (Secale cereale L.). Theor. Appl. Genet. 96 (1998) 203–208.

    Article  CAS  Google Scholar 

  6. Börner, A. and Korzun, V. A consensus linkage map of rye (Secale cereale L.) including 374 RFLPs, 24 isozymes and 15 gene loci. Theor. Appl. Genet. 97 (1998) 1279–1288.

    Article  Google Scholar 

  7. Korzun, V., Malyshev, S., Voylokov, A.V. and Börner, A. A genetic map of rye (Secale cereale L.) combining RFLP, isozyme, protein, microsatellite and gene loci. Theor. Appl. Genet. 102 (2001) 709–717.

    Article  CAS  Google Scholar 

  8. Saal, B. and Wricke, G. Clustering of amplified fragment lenght polymorphism markers in a linkage map of rye (Secale cereale L.). Plant Breed. 121 (2002) 117–123.

    Article  CAS  Google Scholar 

  9. Senft, P. and Wricke, G. An extended genetic map of rye (Secale cereale L.). Plant Breed. 115 (1996) 508–510.

    Article  Google Scholar 

  10. Ma, X-F, Wanous, M.K., Houchins, K., Rodriguez Milla, M.A., Goicoechea, P.G. and Wang Z., Molecular linkage mapping in rye (Secale cereale L.). Theor. Appl. Genet. 102 (2001) 517–523.

    Article  CAS  Google Scholar 

  11. Hackauf, B. and Wehling, P. Development of microsatellite markers in rye: map construction. Plant Breed. Seed Sci. 48 (2003) 143–151.

    Google Scholar 

  12. Khlestkina, E.K., Than, M.H.M., Pestsova, E.G., Röder, M.S., Malyshev, S.V., Korzun, V. and Börner A. Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequence tags. Theor. Appl. Genet. 109 (2004) 725–732.

    PubMed  Article  CAS  Google Scholar 

  13. Milczarski, P., Banek-Tabor, A., Lebiecka, K., Stojałowski, S., Myśków, B. and Masojć P., New genetic map of rye composed of PCR-based molecular markers and its alignment with the reference map of the DS2 × RXL10 intercross. J. Appl. Genet. 48 (2007) 11–24.

    PubMed  Google Scholar 

  14. Börner, A., Korzun, V., Voylokov, A.V., Worland, A.J. and Weber, W.E. Genetic mapping of quantitative trait loci in rye (Secale cereale L.). Euphytica 116 (2000) 203–209.

    Article  Google Scholar 

  15. Miedaner, T., Glass, C., Dreyer, F., Wilde, P., Wortmann, H. and Geiger H.H. Mapping of genes for male-fertility restoration in ‘Pampa’ CMS winter rye (Secale cereale L.). Theor. Appl. Genet. 101 (2000) 1226–1233.

    Article  CAS  Google Scholar 

  16. Miftahudin, Scoles, G.J. and Gustafson, J.P. AFLP markers tightly linked to the aluminium-tolerance gene Alt3 in rye (Secale cereale L.) Theor. Appl. Genet. 104 (2002) 626–631.

    Article  CAS  Google Scholar 

  17. Milczarski, P. and Masojć, P. Interval mapping of genes controlling growth of rye plants. Plant Breed. Seed Sci. 48 (2003) 135–142.

    Google Scholar 

  18. Masojć, P. and Milczarski P. Mapping QTLs for alpha-amylase activity in rye grain. J. Appl. Genet. 46 (2005) 115–123.

    PubMed  Google Scholar 

  19. Masojć, P., Banek-Tabor, A., Milczarski, P. and Twardowska, M. QTLs for resistance to preharvest sprouting in rye (Secale cereale L.) J. Appl. Genet. 48 (2007) 211–217.

    PubMed  Google Scholar 

  20. Somers, D.J., Isaac, P. and Edwards K. A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.) Theor. Appl. Genet. 109 (2004) 1105–1114.

    PubMed  Article  CAS  Google Scholar 

  21. Wenzl, P., Li, H., Carling, J., Zhou, M., Raman, H., Paul, E., Hearnden, P., Maier, Ch., Xia, L., Caig, V., Ovesna, J., Cakir, M., Poulsen, D., Wang, J., Raman, R., Smith, K.P., Muehlbauer, G.J., Chalmers, K.J., Kleinhofs, A., Huttner, E. and Kilian, A. A high density consensus map of barley linking DarT markers to SSR, RFLP and STS loci and agricultural traits. BMC Genomics 7 (2006) 206.

    PubMed  Article  Google Scholar 

  22. Hearnden, P.R., Eckermann, P.J., McMichael, G.L., Hayden, M.J., Eglinton, J.K. and Chalmers K.J. A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theor. Appl. Genet. 115 (2007) 383–391.

    PubMed  Article  CAS  Google Scholar 

  23. Myśków, B., Masojć, P., Banek-Tabor, A. and Szołkowski A. Genetic diversity of inbred rye lines evaluated by RAPD analysis. J. Appl. Genet. 42 (2001) 1–14.

    PubMed  Google Scholar 

  24. Masojć, P., Myśków, B. and Milczarski P. Extending a RFLP-based genetic map of rye using random amplified polymorphic DNA (RAPD) and isozyme markers. Theor. Appl. Genet. 102 (2001) 1273–1279.

    Article  Google Scholar 

  25. Bednarek, P.T., Masojć, P., Lewandowska, R. and Myśków, B. Saturating rye genetic map with amplified fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) markers. J. Appl. Genet. 44 (2003) 21–33.

    PubMed  Google Scholar 

  26. Stojałowski, S., Milczarski, P. and Masojć, P. Usefulness of ISSR markers for identification of inbred lines and mapping of rye genome. Biul. Inst. Hod. Aklim. Rośl. 231 (2004) 237–246.

    Google Scholar 

  27. Stojałowski, S., Jaciubek, M., Masojć, P. Rye SCAR markers for male fertility restoration in the P cytoplasm are also applicable to marker-assisted selection in the C cytoplasm. J. Appl. Genet. 46 (2005) 371–373.

    PubMed  Google Scholar 

  28. Van Ooijen, J.W., Voorrips, R.E., JoinMap® v. 3.0, Software for the calculation of genetic linkage maps. Plant Research International B.V. Wageningen, the Netherlands, 2001.

    Google Scholar 

  29. Doligez, A., Adam-Blondon, A.F., Cipriani, G., Di Gaspero, G., Laucou, V., Merdinoglu, D., Meredith, C.P., Riaz, S., Roux, C. and This, P. An itegrated SSR map of grapevine based on five mapping populations Theor. Appl. Genet. 113 (2006) 369–382.

    PubMed  Article  CAS  Google Scholar 

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Correspondence to Beata Myśków.

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Stojałowski, S., Myśków, B., Milczarski, P. et al. A consensus map of chromosome 6R in rye (Secale cereale L.). Cell Mol Biol Lett 14, 190–198 (2009). https://doi.org/10.2478/s11658-008-0042-5

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  • DOI: https://doi.org/10.2478/s11658-008-0042-5

Key words

  • Rye
  • Consensus map
  • Molecular markers