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  • Short Communication
  • Open Access

The ceramide structure of GM1 ganglioside differently affects its recovery in low-density membrane fractions prepared from HL-60 cells with or without triton-X100

  • 1,
  • 1,
  • 2,
  • 1,
  • 2 and
  • 1Email author
Cellular & Molecular Biology LettersAn International Journal200814:175

https://doi.org/10.2478/s11658-008-0043-4

  • Received: 19 March 2008
  • Accepted: 11 July 2008
  • Published:

Abstract

Gangliosides are characteristically enriched in various membrane domains that can be isolated as low density membrane fraction insoluble in detergents (detergent-resistant membranes, DRMs) or obtained after homogenization and sonication in 0.5 M sodium carbonate (low-density membranes, LDMs). We assessed the effect of the ceramide structure of four [3H]-labeled GM1 ganglioside molecular species (GM1s) taken up by HL-60 cells on their occurrence in LDMs, and compared it with our previous observations for DRMs. All GM1s contained C18 sphingosine, which was acetylated in GM1(18:1/2) or acylated with C14, C18 or C18:1 fatty acids (Fas)

Keywords

  • Ceramide
  • Gangliosides
  • GM1
  • Membrane domains
  • Myristic acid
  • Sonication

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