Open Access

The ceramide structure of GM1 ganglioside differently affects its recovery in low-density membrane fractions prepared from HL-60 cells with or without triton-X100

  • Mirosława Panasiewicz1,
  • Hanna Domek1,
  • Grażyna Hoser2,
  • Natalia Fedoryszak1,
  • Maciej Kawalec2 and
  • Tadeusz Pacuszka1Email author
Cellular & Molecular Biology LettersAn International Journal200814:175

https://doi.org/10.2478/s11658-008-0043-4

Received: 19 March 2008

Accepted: 11 July 2008

Published: 31 October 2008

Abstract

Gangliosides are characteristically enriched in various membrane domains that can be isolated as low density membrane fraction insoluble in detergents (detergent-resistant membranes, DRMs) or obtained after homogenization and sonication in 0.5 M sodium carbonate (low-density membranes, LDMs). We assessed the effect of the ceramide structure of four [3H]-labeled GM1 ganglioside molecular species (GM1s) taken up by HL-60 cells on their occurrence in LDMs, and compared it with our previous observations for DRMs. All GM1s contained C18 sphingosine, which was acetylated in GM1(18:1/2) or acylated with C14, C18 or C18:1 fatty acids (Fas)

Keywords

CeramideGangliosidesGM1Membrane domainsMyristic acidSonication

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