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Acheron, an novel LA antigen family member, binds to cask and forms a complex with id transcription factors

An Erratum to this article was published on 28 January 2009

Abstract

Acheron, a Lupus antigen ortholog, was identified as a novel death-associated transcript from the intersegmental muscles of the mothManduca sexta. Acheron is phylogenetically-conserved and represents a new sub-family of Lupus antigen proteins. Acheron is expressed predominantly in neurons and muscle in vertebrates, and regulates several developmental events including myogenesis, neurogenesis and possibly metastasis. Using Acheron as bait, we performed a yeast two-hybrid screen with a mouse embryo cDNA library and identified CASK-C, a novel CASK/Lin-2 isoform, as an Acheron binding partner. Acheron and CASK-C bind via the C-terminus of Acheron and the CaMKII-like domain of CASK-C. Co-immunoprecipitation assays verify this interaction and demonstrate that Acheron also forms a complex with all members of the Id (inhibitor of differentiation) proteins. Taken together, these data suggest a mechanism by which Acheron may regulate development and pathology.

Abbreviations

Achn:

Acheron

CASK:

Ca2+/calmodulin-dependent serine protein kinase

GST:

glutathione S transferase

Id:

inhibitor of differentiation

LA:

Lupus antigen

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Correspondence to Lawrence M. Schwartz.

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An erratum to this article can be found at http://dx.doi.org/10.2478/s11658-009-0005-5

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Weng, H., Kim, C., Valavanis, C. et al. Acheron, an novel LA antigen family member, binds to cask and forms a complex with id transcription factors. Cell Mol Biol Lett 14, 273–287 (2009). https://doi.org/10.2478/s11658-008-0046-1

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  • DOI: https://doi.org/10.2478/s11658-008-0046-1

Key words

  • Programmed cell death
  • Manduca sexta
  • Muscle
  • Apoptosis
  • Lupus antigen protein