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The expression of COX-2, hTERT, MDM2, LATS2 and S100A2 in different types of non-small cell lung cancer (NSCLC)


Several studies have reported different expression levels of certain genes in NSCLC, mostly related to the stage and advancement of the tumours. We investigated 65 stage I-III NSCLC tumours: 32 adenocarcinomas (ADC), 26 squamous cell carcinomas (SCC) and 7 large cell carcinomas (LCC). Using the real-time reverse transcription polymerase chain reaction (RT-PCR), we analysed the expression of the COX-2, hTERT, MDM2, LATS2 and S100A2 genes and researched the relationships between the NSCLC types and the differences in expression levels. The differences in the expression levels of the LATS2, S100A2 and hTERT genes in different types of NSCLC are significant. hTERT and COX-2 were over-expressed and LATS2 under-expressed in all NSCLC. We also detected significant relative differences in the expression of LATS2 and MDM2, hTERT and MDM2 in different types of NSCLC. There was a significant difference in the average expression levels in S100A2 for ADC and SCC. Our study shows differences in the expression patterns within the NSCLC group, which may mimic the expression of the individual NSCLC type, and also new relationships in the expression levels for different NSCLC types.





cyclin-dependent kinase 2


complementary DNA


cyclooxygenase 2

CT :

cycle threshold


deoxyribonucleic acid


glyceraldehyde-3-phosphate dehydrogenase


human telomerase reverse transcriptase




homolog of large tumour suppressor 2, Drosophilae


large cell carcinoma


mouse double minute 2 homolog


messenger RNA


non-small cell lung cancer


cyclin-dependent kinase inhibitor 1A


tumour protein p53


polymerase chain reaction


pathological tumour-node-metastasis




ribonucleic acid

rs :

Spearman’s rank correlation coefficient


real time PCR


S100 calcium binding protein A2


squamous cell carcinoma


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Correspondence to Damjan Glavač.

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Stražišar, M., Mlakar, V. & Glavač, D. The expression of COX-2, hTERT, MDM2, LATS2 and S100A2 in different types of non-small cell lung cancer (NSCLC). Cell Mol Biol Lett 14, 442–456 (2009).

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Key words

  • COX-2
  • hTERT
  • MDM2
  • LATS2
  • S100A2
  • RT-PCR
  • Expression