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The expression of COX-2, hTERT, MDM2, LATS2 and S100A2 in different types of non-small cell lung cancer (NSCLC)

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Abstract

Several studies have reported different expression levels of certain genes in NSCLC, mostly related to the stage and advancement of the tumours. We investigated 65 stage I-III NSCLC tumours: 32 adenocarcinomas (ADC), 26 squamous cell carcinomas (SCC) and 7 large cell carcinomas (LCC). Using the real-time reverse transcription polymerase chain reaction (RT-PCR), we analysed the expression of the COX-2, hTERT, MDM2, LATS2 and S100A2 genes and researched the relationships between the NSCLC types and the differences in expression levels. The differences in the expression levels of the LATS2, S100A2 and hTERT genes in different types of NSCLC are significant. hTERT and COX-2 were over-expressed and LATS2 under-expressed in all NSCLC. We also detected significant relative differences in the expression of LATS2 and MDM2, hTERT and MDM2 in different types of NSCLC. There was a significant difference in the average expression levels in S100A2 for ADC and SCC. Our study shows differences in the expression patterns within the NSCLC group, which may mimic the expression of the individual NSCLC type, and also new relationships in the expression levels for different NSCLC types.

Abbreviations

ADC:

adenocarcinoma

CDK2:

cyclin-dependent kinase 2

cDNA:

complementary DNA

COX-2:

cyclooxygenase 2

CT :

cycle threshold

DNA:

deoxyribonucleic acid

GAPDH:

glyceraldehyde-3-phosphate dehydrogenase

hTERT:

human telomerase reverse transcriptase

IHC:

immunohistochemistry

LATS2:

homolog of large tumour suppressor 2, Drosophilae

LCC:

large cell carcinoma

MDM2:

mouse double minute 2 homolog

mRNA:

messenger RNA

NSCLC:

non-small cell lung cancer

p21:

cyclin-dependent kinase inhibitor 1A

p53:

tumour protein p53

PCR:

polymerase chain reaction

pTNM:

pathological tumour-node-metastasis

RB:

retinoblastoma

RNA:

ribonucleic acid

rs :

Spearman’s rank correlation coefficient

RT-PCR:

real time PCR

S100A2:

S100 calcium binding protein A2

SCC:

squamous cell carcinoma

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Correspondence to Damjan Glavač.

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Key words

  • COX-2
  • hTERT
  • MDM2
  • LATS2
  • S100A2
  • RT-PCR
  • Expression