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The expression of protein kinase B in gastric cancer cell apoptosis induced by 12-O-tetradecanoylphorbol-1, 3-acetate

Abstract

Protein kinase B (PKB/Akt) is a serine-threonine kinase functioning downstream of phosphatidylinositol 3-kinase (PI-3 kinase) in response to mitogen or growth factor stimulation. In several cell types, it plays an important anti-apoptotic role. TPA is a potent regulator of the growth of many different cell types. Here, we detected that TPA could induce cell apoptosis in the gastric cancer cell line, BGC-823. We also found that TPA inhibited the expression of PKB/Akt in a TPA concentration- and time-dependent manner. Furthermore, TPA inhibited the phosphorylation of PKB at Ser473, but did not affect the phosphorylation of Thr308. It only attenuated the expression of PKB/Akt and the phosphorylation of Ser473 in the cell nucleus, whereas it did not change the PKB/Akt distribution in BGC-823 cells. These results suggest that PKB/Akt inhibition by TPA may be the important factor in the mechanism of effect of TPA on gastric cell lines.

Abbreviations

BrdU:

5-bromo-2′-deoxyuridine

OA:

okadaic acid

PDK1/PDK2:

3-phosphoinositide-dependent kinase

PI:

propidium iodide

PI3K:

phosphoinositide-3-OH kinase

PKB/Akt:

protein kinase B

TPA:

12-O-tetradecanoylphorbol-1,3-acetate

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Correspondence to Chun Xia.

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Zhang, B., Xia, C. The expression of protein kinase B in gastric cancer cell apoptosis induced by 12-O-tetradecanoylphorbol-1, 3-acetate. Cell Mol Biol Lett 14, 466–480 (2009). https://doi.org/10.2478/s11658-009-0013-5

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  • DOI: https://doi.org/10.2478/s11658-009-0013-5

Key words

  • TPA
  • Cell apoptosis
  • PKB/Akt
  • Gastric cancer BGC-823 cell line