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Overexpression of human osteopontin increases cell proliferation and migration in human embryo kidney-293 cells

Abstract

Malignant tumors are characterized by dysregulated cell growth and the metastasis of secondary tumors. Numerous studies have documented that osteopontin (OPN) plays a key role in regulating tumor progression and metastasis. Here, we show that the overexpression of OPN in human embryo kidney-293 cells significantly increases both the level of cell proliferation, by provoking the G1/S transition, and the level of cell migration in vitro. These findings suggest that augmented OPN contributes to cell growth and motility. Inhibiting OPN or the pathway it stimulates may therefore represent a novel approach for the treatment of primary tumors and associated metastases.

Abbreviations

ECM:

extracellular matrix

hOPN:

human OPN

IRES:

internal ribosome entry site

OPN:

osteopontin

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Correspondence to Ya-Jun Liu or Wei Tian.

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Key words

  • Osteopontin
  • Overexpression
  • Proliferation
  • Metastasis