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Overexpression of human osteopontin increases cell proliferation and migration in human embryo kidney-293 cells


Malignant tumors are characterized by dysregulated cell growth and the metastasis of secondary tumors. Numerous studies have documented that osteopontin (OPN) plays a key role in regulating tumor progression and metastasis. Here, we show that the overexpression of OPN in human embryo kidney-293 cells significantly increases both the level of cell proliferation, by provoking the G1/S transition, and the level of cell migration in vitro. These findings suggest that augmented OPN contributes to cell growth and motility. Inhibiting OPN or the pathway it stimulates may therefore represent a novel approach for the treatment of primary tumors and associated metastases.



extracellular matrix


human OPN


internal ribosome entry site




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Correspondence to Ya-Jun Liu or Wei Tian.

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Liu, YJ., Zhang, DQ., Sui, XM. et al. Overexpression of human osteopontin increases cell proliferation and migration in human embryo kidney-293 cells. Cell Mol Biol Lett 14, 670–678 (2009).

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Key words

  • Osteopontin
  • Overexpression
  • Proliferation
  • Metastasis