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The intrabody targeting of hTERT attenuates the immortality of cancer cells


hTERT (human telomerase reverse transcriptase) plays a key role in the process of cell immortalization. Overexpression of hTERT has been implicated in 85% of malignant tumors and offers a specific target for cancer therapy. In this paper, we describe an effective approach using a single-chain variable fragment (scFv) intrabody derived from monoclonal hybridoma directed against hTERT to attenuate the immortalization of human uterine cervix and hepatoma cells. The scFv we constructed had a high affinity to hTERT, and specifically neutralized over 70% of telomere synthesis activity, thereby inhibiting the viability and proliferation of the cancer cells. Our results indicate that this anti-hTERT intrabody is a promising tool to target hTERT and intervene in the immortalization process of cancer cells.





enhanced chemiluminescence


enzyme linked immunosorbent assay


fluorescein isothiocyanate


horseradish peroxidase


monoclonal antibody


mouse intraperitoneal fluid




propidium iodide


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Correspondence to Daming Ren.

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Zhu, X., Yang, N., Cai, J. et al. The intrabody targeting of hTERT attenuates the immortality of cancer cells. Cell Mol Biol Lett 15, 32 (2010).

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Key words

  • Cancer
  • Intrabody
  • Anti-hTERT ScFv
  • Immortality