- Research Article
The intrabody targeting of hTERT attenuates the immortality of cancer cells
Cellular & Molecular Biology Letters volume 15, Article number: 32 (2010)
hTERT (human telomerase reverse transcriptase) plays a key role in the process of cell immortalization. Overexpression of hTERT has been implicated in 85% of malignant tumors and offers a specific target for cancer therapy. In this paper, we describe an effective approach using a single-chain variable fragment (scFv) intrabody derived from monoclonal hybridoma directed against hTERT to attenuate the immortalization of human uterine cervix and hepatoma cells. The scFv we constructed had a high affinity to hTERT, and specifically neutralized over 70% of telomere synthesis activity, thereby inhibiting the viability and proliferation of the cancer cells. Our results indicate that this anti-hTERT intrabody is a promising tool to target hTERT and intervene in the immortalization process of cancer cells.
enzyme linked immunosorbent assay
mouse intraperitoneal fluid
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Zhu, X., Yang, N., Cai, J. et al. The intrabody targeting of hTERT attenuates the immortality of cancer cells. Cell Mol Biol Lett 15, 32 (2010). https://doi.org/10.2478/s11658-009-0032-2
- Anti-hTERT ScFv