Open Access

Global mapping of ZBTB7A transcription factor binding sites in HepG2 cells

  • Xuyu Zu1, 4,
  • Lingling Yu1, 4,
  • Yiming Sun3, 4,
  • Jing Tian1, 4,
  • Feng Liu1, 4,
  • Qinsheng Sun1, 4,
  • Shengnan He1, 4,
  • Guang Sun1, 4,
  • Weishi Luo1, 4 and
  • Yuyang Jiang1, 2, 4Email author
Cellular & Molecular Biology LettersAn International Journal201015:3

https://doi.org/10.2478/s11658-010-0003-7

Received: 30 August 2009

Accepted: 17 February 2010

Published: 19 March 2010

Abstract

ZBTB7A is a known proto-oncogene that is implicated in carcinogenesis and cell differentiation and development. Fully understanding the function of ZBTB7A in cellular processes could provide useful strategies for cancer treatment and development-associated disease therapy. Here, global mapping of ZBTB7A transcription factor binding sites was developed by utilizing microarray technology in HepG2 cells. The data obtained from the microarrays was further validated via chromatin immunoprecipitation-PCR (ChIP-PCR) and real time-PCR, and it was revealed that ZBTB7A may be one of the regulators of neural development. ZBTB7A target signal pathways were identified in signal pathway and GO (Gene Ontology) analyses. This is the first report on the global mapping of ZBTB7A downstream direct targets, and these findings will be useful in understanding the roles of ZBTB7A in cellular processes.

Key words

ZBTB7A Proto-oncogene Transcription factor ChIP-on-chip analysis Signal pathway

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