Open Access

The anti-apoptotic protein lifeguard is expressed in breast cancer cells and tissues

  • Vesna Bucan1, 2Email author,
  • Kerstin Reimers1,
  • Claudia Yu Choi1,
  • Mau-Thek Eddy1 and
  • Peter M. Vogt1
Cellular & Molecular Biology LettersAn International Journal201015:9

https://doi.org/10.2478/s11658-010-0009-1

Received: 7 October 2009

Accepted: 4 March 2010

Published: 19 March 2010

Abstract

Lifeguard (LFG) is an anti-apoptotic protein that inhibits Fas-mediated death in tumour cells. However, the molecular function of human LFG in the carcinogenesis of human breast cells is uncertain. We studied the expression and function of endogenous LFG in four breast cancer cell lines (MCF-7, MDA-MB-231, T-47D and HS 578T), a human breast epithelial cell line (HS 578Bst), and in healthy and cancerous breast tissues. Molecular (Western blot and RT-PCR) and immunohistochemical techniques were used to investigate the LFG expression. To investigate the breast cancer cell proliferation in the presence of Fas, we performed fluorescent cell viability assays. The possible association of Fas with LFG was analyzed by immunofluorescence microscopy. In this paper, we provide convincing evidence that LFG is overexpressed in several human breast cancer cell lines. More importantly, we found that the LFG expression correlates with high tumour grades in primary breast tumours. Finally, we demonstrated that Fas sensitivity is reduced in breast cancer cell lines expressing LFG. Our results indicated that LFG is strongly expressed in breast cancer epithelial cells. Moreover, the overexpression of LFG correlated with tumour grade and reduced Fas sensitivity. Our findings support the idea that LFG may have a role in the downregulation of apoptosis in breast cancer cells.

Key words

Apoptosis Lifeguard Fas Breast cancer

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