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The heterogeneity of cell subtypes from a primary culture of human amniotic fluid

Cellular & Molecular Biology Letters volume 15, pages 424–439 (2010)Cite this article

Abstract

Heterogeneous human amniotic fluid contains various cell types. Herein, we report on the possibility of simultaneously isolating three subtypes of cells from one primary culture. Using a stainless steel instrument named a colony poculum, two of the three cell subtypes could be efficiently cultured, and these were further characterized. The results indicated that these two cell subtypes had different morphologies and were characterized by different cell marker expression profiles, including the differential expression of CD105, CD117 and EBAF. Furthermore, their gene expression array data revealed their different gene expression profiles. Although both cell types expressed several embryonic stem cell-specific markers, they were non-tumorigenic in vivo. This paper not only provides new insight into the heterogeneity of human amniotic fluid, it also presents a simple yet efficient cell isolation method. These results will contribute to the thorough investigation of the properties and potential future applications of human amniotic fluid-derived cells.

Abbreviations

AF-type:

amniotic fluid-specific

DAPI:

dipeptidyl aminopeptidase

EC cells:

embryonal carcinoma cells

EG cells:

embryonic germ cells

ES cells:

embryonic stem cells

E-type:

epitheloid type

FITC:

fluorescein isothiocyanate

F-type:

fibroblastic type

GO:

gene ontology

hAFC:

human amniotic fluid-derived cells

PBS:

phosphate-buffered saline

PE:

phycoerythrin

RT:

room temperature

RT-PCR:

reverse transcriptase polymerase chain reaction

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Authors and Affiliations

  1. Department of Urology, Shanghai Children’s Hospital, Shanghai Jiao Tong University School of Medicine, 1400 Beijing Road West, Shanghai, 200040, China

    Shengli Zhang, Hua Xie, Qiquan Wu, Xiaorong Ma, Junmei Zhou & Fang Chen

  2. Department of Urology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

    Shengli Zhang, Qiquan Wu & Xiaorong Ma

  3. Tissue Engineering Laboratory, Xinhua Hospital, Shanghai Institute for Pediatric Research, Shanghai Jiao Tong University School of Medicine, Shanghai, China

    Hongquan Geng, Junmei Zhou & Fang Chen

Authors
  1. Shengli Zhang
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  2. Hongquan Geng
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  3. Hua Xie
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  4. Qiquan Wu
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  5. Xiaorong Ma
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  6. Junmei Zhou
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  7. Fang Chen
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Correspondence to Junmei Zhou or Fang Chen.

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Zhang, S., Geng, H., Xie, H. et al. The heterogeneity of cell subtypes from a primary culture of human amniotic fluid. Cell Mol Biol Lett 15, 424–439 (2010). https://doi.org/10.2478/s11658-010-0017-1

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  • DOI: https://doi.org/10.2478/s11658-010-0017-1

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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