- Short Communication
Gender dimorphism in the exercise-naïve murine skeletal muscle proteome
Cellular & Molecular Biology Lettersvolume 15, pages507–516 (2010)
Skeletal muscle is a plastic tissue with known gender dimorphism, especially at the metabolic level. A proteomic comparison of male and female murine biceps brachii was undertaken, resolving an average of 600 protein spots of MW 15–150 kDa and pI 5–8. Twenty-six unique full-length proteins spanning 11 KOG groups demonstrated statistically significant (p<0.05) abundance differences between genders; the majority of these proteins have metabolic functions. Identified glycolytic enzymes demonstrated decreased abundance in females, while abundance differences in identified oxidative phosphorylation enzymes were specific to the proteins rather than to the functional group as a whole. Certain cytoskeletal and stress proteins showed specific expression differences, and all three phosphorylation states of creatine kinase showed significant decreased abundance in females. Expression differences were significant but many were subtle (≤ 2-fold), and known hormonally-regulated proteins were not identified. We conclude that while gender dimorphism is present in non-exercised murine skeletal muscle, the proteome comparison of male and female biceps brachii in exercise-naive mice indicates subtle differences rather than a large or obviously hormonal dimorphism.
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Tarnopolsky, M.A. Gender differences in carbohydrate loading are related to energy intake. J. Appl. Physiol. 91 (2001) 225–230.
Phillips, S.M., Atkinson, S.A., Tarnopolsky, M.A. and MacDougall, J.D. Gender differences in leucine kinetics and nitrogen balance in endurance athletes. J. Appl. Physiol. 75 (1993) 2134–2141.
Tarnopolsky, M.A. Sex differences in exercise metabolism and the role of 17-β estradiol. Med. Sci. Sports Exerc. 40 (2008) 648–654.
Metskas, L.A., Kulp, M. and Scordilis, S.P. Differential proteome profiling analysis of male and female murine skeletal muscle following a repeat bout of exercise. FASEB J. 23 (2009): 601.4.
Yoshioka, M., Boivin, A., Bolduc, C. and St-Amand, J. Gender difference of androgen actions on skeletal muscle transcriptome. J. Mol. Endocrinol. 39 (2007) 119–133.
Roth, S.M., Ferrell, R.E., Peters, D.G., Metter, E.J., Hurley, B.F. and Rogers, M.A. Influence of age, sex, and strength training on human muscle gene expression determined by microarray. Physiol. Genomics 10 (2002) 181–190.
Thompson, H.S. When stress is good: exercise and stress protein responses in mice and humans. PhD dissertation, University of Massachusetts Amherst, Amherst, Massachusetts, (2003).
Thompson, H.S, Clarkson, P.M., and Scordilis, S.P. The repeated bout effect and heat shock proteins: intramuscular HSP27 and HSP70 expression following two bouts of eccentric exercise in humans. Acta Physiol. Scand. 174 (2002) 47–56.
Lowry, O.H., Rosenburg, N.J., Farr, A.L. and Randall, R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193 (1951) 265–275.
Bio-Rad Laboratories. ReadyPrep 2-D Starter Kit Instruction Manual. Cat. No. 163-2105.
Colom, B., Alcolea, M.P., Valle, A., Oliver, J., Roca, P. and Garcia-Palmer, F.J. Skeletal muscle of female rats exhibit higher mitochondrial mass and oxidative-phosphorylative capacities compared to males. Cell. Physiol. Biochem. 19 (2007) 205–212.
Izawa, I. and Inagaki, M. Regulatory mechanisms and functions of intermediate filaments: a study using site- and phosphorylation state-specific antibodies. Cancer Sci. 93 (2006) 167–174.
Zhou, H., Huiatt, T.W., Robson, R.M., Sernett, S.W., and D.J. Graves. Characterization of ADP-ribosylation sites on desmin and restoration of desmin intermediate filament assembly by de-ADP-ribosylation. Arch. Biochem. Biophys. 334 (1996) 214–222.
Amelink, G.J., Kamp, H.H. and Bar, P.R. Creatine kinase isoenzyme profiles after exercise in the rat: sex-linked differences in leakage of CKMM. Pflugers Arch. 412 (1988) 417–421.
Norton, J.P., Clarkson, P.M., Graves, J.E., Litchfield, P.L. and Kirwan, J. Serum creatine kinase activity and body composition in males and females. Hum. Biol. 57 (1985) 591–598.
Kendall, B. and Eston, R. Exercise-induced muscle damage and the potential protective role of estrogen. Sports Med. 32 (2002) 103–123.
Rune, A., Salehzadeh, F., Szekeres, F., Kuhn, I., Osler, M.E. and Al-Khalili, L. Evidence against a sexual dimorphism in glucose and fatty acid metabolism in skeletal muscle cultures from age-matched men and postmenopausal women. Acta Physiol. 197 (2009) 207–215.
Hamadeh, M.J., Devries, M.C. and Tarnopolsky, M.A. Estrogen supplementation reduces whole body leucine and carbohydrate oxidation and increases lipid oxidation in men during endurance exercise. J. Clin. Endocrinol. Metab. 90 (2005) 3592–3599.
Barros, R.P.A., Machado, U.F., Warner, M. and Gustafsson, J.A. Muscle GLUT4 regulation by estrogen receptors ERβ and ERβ. PNAS 103 (2006) 1605–1608.
Devries, M.C., Hamadeh, M.J., Phillips, S.M. and Tarnopolsky, M.A. Menstrual cycle phase and sex influence muscle glycogen utilization and glucose turnover during moderate-intensity endurance exercise. Am. J. Physiol. Regulatory Integrative Comp. Physiol. 291 (2006) 1120–1128.