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ZNF300, a recently identified human transcription factor, activates the human IL-2Rβ promoter through the overlapping ZNF300/EGR1 binding site

Cellular & Molecular Biology Letters volume 15, pages 530–540 (2010)Cite this article

Abstract

ZNF300 was recently identified as a member of the human KRAB/C2H2 zinc finger protein family. Little is known about the role of ZNF300 in human gene regulation networks. In this study, the DNA-binding property of ZNF300 was further analyzed. We found that the recombinant ZNF300 could bind to the binding site 5′-GCGGGGGCG-3′ of Egr1, another member of the KRAB/C2H2 zinc finger protein family. Similarly, recombinant Egr1 also showed a similar binding affinity to the ZNF300 binding site 5′-CTGGGGGCG-3′. Bioinformatics analysis revealed that there is an overlapping ZNF300/Egr1 binding site in the human IL-2Rβ promoter region, which was previously known to be recognized by endogenous Egr1. Electrophoretic mobility shift assays showed that endogenous ZNF300 could also bind to this site. A transient transfection assay revealed that both ZNF300 and Egr1 could transactivate the IL-2Rβ promoter, and that the activation was abrogated by a mutation of residues in the overlapping ZNF300/Egr1 binding site. Co-expression of ZNF300 and Egr1 led to enhanced IL-2Rβ promoter activity. Thus, ZNF300 is likely to be another regulator of the human IL-2Rβ promoter.

Abbreviations

EMSA:

electrophoretic mobility shift assay

IL-2Rβ:

IL-2 receptor beta chain

KRAB:

Krüppel-asscociated box

TPA:

12-o-tetradecanoylphorbol-13-acetate

ZNF300:

zinc finger 300

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Authors and Affiliations

  1. State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, P.R. China

    Lu Xue, Hongling Qiu, Jian Ma, Mingxiong Guo & Wenxin Li

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  1. Lu Xue
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  2. Hongling Qiu
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  3. Jian Ma
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  4. Mingxiong Guo
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  5. Wenxin Li
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Correspondence to Wenxin Li.

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These authors contributed equally to this work

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Xue, L., Qiu, H., Ma, J. et al. ZNF300, a recently identified human transcription factor, activates the human IL-2Rβ promoter through the overlapping ZNF300/EGR1 binding site. Cell Mol Biol Lett 15, 530–540 (2010). https://doi.org/10.2478/s11658-010-0025-1

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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