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TRPC expression in mesenchymal stem cells

Cellular & Molecular Biology Letters volume 15, pages 600–610 (2010)Cite this article

Abstract

Transient receptor potential canonical (TRPC) channels are key players in calcium homeostasis and various regulatory processes in cell biology. Little is currently known about the TRPC subfamily members in mesenchymal stem cells (MSC), where they could play a role in cell proliferation. We report on the presence of TRPC1, 2, 4 and 6 mRNAs in MSC. Western blot and immunofluorescence staining indicate a membrane and intracellular distribution of TRPC1. Furthermore, the decrease in the level of TRPC1 protein caused by RNA interference is accompanied by the downregulation of cell proliferation. These results indicate that MSC express TRPC1, 2, 4 and 6 mRNA and that TRPC1 may play a role in stem cell proliferation.

Abbreviations

MSC:

Mesenchymal stem cells

TRP:

Transient receptor potential

TRPC:

Transient receptor potential canonical

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Authors and Affiliations

  1. IFRMP, Institute for Biomedical Research, Inserm, U905, University of Rouen, Rouen, France

    Frederic Torossian, Aurelie Bisson, Olivier Boyer & Marek Lamacz

  2. Institute for Biomedical Research, EA 3829, University of Rouen, Rouen, France

    Frederic Torossian & Jean-Pierre Vannier

  3. Faculty of Medicine and Pharmacy, Inserm U905, 22 bd Gambetta, F-76000, Rouen, France

    Marek Lamacz

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  1. Frederic Torossian
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  2. Aurelie Bisson
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Correspondence to Marek Lamacz.

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Torossian, F., Bisson, A., Vannier, JP. et al. TRPC expression in mesenchymal stem cells. Cell Mol Biol Lett 15, 600–610 (2010). https://doi.org/10.2478/s11658-010-0031-3

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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