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A functional analysis of G23A polymorphism and the alternative splicing in the expression of the XPA gene

Cellular & Molecular Biology Letters volume 15, pages 611–629 (2010)Cite this article

Abstract

The XPA gene has a commonly occurring polymorphism (G23A) associated with cancer risk. This study assessed the functional significance of this polymorphism, which is localised near the translation start codon. Lymphoblastoid cell lines with alternative homozygous genotypes showed no significant differences in their XPA levels. The luciferase reporter assay detected no functional difference between the two sequences. Unexpectedly, we found that the alternatively spliced form of XPA mRNA lacked a part of exon 1. Only the reading frame downstream of codon Met59 was preserved. The alternative mRNA is expressed in various human tissues. The analysis of the 5’cDNA ends showed similar transcription start sites for the two forms. The in vitro expression of the alternative XPA labelled with the red fluorescent protein (mRFP) showed a lack of preferential nuclear accumulation of the XPA isoform. The biological role of the alternative XPA mRNA form remains to be elucidated.

Abbreviations

DAPI:

4’,6-diamidino-2-phenylindole

DMEM:

Dulbecco’s Modified Eagle Medium

DMSO:

dimethylsulfoxide

dNTP:

deoxyribonucleotide triphosphate

GAPDH:

glyceraldehyde-3-phosphate dehydrogenase

mRFP:

monomeric red fluorescent protein

PCR:

polymerase chain reaction

RACE:

rapid amplification of cDNA ends

RPA:

replication protein A

SNP:

single nucleotide polymorphism

TDG:

thymine-DNA glycosylase

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Authors and Affiliations

  1. Department of Tumour Biology, Maria Skłodowska-Curie Memorial Cancer Centre and Institute of Oncology, Gliwice Branch, Wybrzeże Armii Krajowej 15, 44-101, Gliwice, Poland

    Dorota Butkiewicz, Małgorzata Krześniak, Rasa Vaitiekunaite, Bożena Sikora & Marek Rusin

  2. Laboratory of Human Carcinogenesis, National Cancer Institute, NIH, 37 Convent Drive, Bethesda, MD, 20892-4258, USA

    Elise D. Bowman & Curtis C. Harris

Authors
  1. Dorota Butkiewicz
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  2. Małgorzata Krześniak
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  3. Rasa Vaitiekunaite
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  4. Bożena Sikora
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  6. Curtis C. Harris
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  7. Marek Rusin
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Correspondence to Marek Rusin.

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Butkiewicz, D., Krześniak, M., Vaitiekunaite, R. et al. A functional analysis of G23A polymorphism and the alternative splicing in the expression of the XPA gene. Cell Mol Biol Lett 15, 611–629 (2010). https://doi.org/10.2478/s11658-010-0032-2

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  • DOI: https://doi.org/10.2478/s11658-010-0032-2

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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