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Exploring the binding dynamics of BAR proteins

Cellular & Molecular Biology Letters volume 16, pages 398–411 (2011)Cite this article

Abstract

We used a continuum model based on the Helfrich free energy to investigate the binding dynamics of a lipid bilayer to a BAR domain surface of a crescent-like shape of positive (e.g. I-BAR shape) or negative (e.g. F-BAR shape) intrinsic curvature. According to structural data, it has been suggested that negatively charged membrane lipids are bound to positively charged amino acids at the binding interface of BAR proteins, contributing a negative binding energy to the system free energy. In addition, the cone-like shape of negatively charged lipids on the inner side of a cell membrane might contribute a positive intrinsic curvature, facilitating the initial bending towards the crescent-like shape of the BAR domain. In the present study, we hypothesize that in the limit of a rigid BAR domain shape, the negative binding energy and the coupling between the intrinsic curvature of negatively charged lipids and the membrane curvature drive the bending of the membrane. To estimate the binding energy, the electric potential at the charged surface of a BAR domain was calculated using the Langevin-Bikerman equation. Results of numerical simulations reveal that the binding energy is important for the initial instability (i.e. bending of a membrane), while the coupling between the intrinsic shapes of lipids and membrane curvature could be crucial for the curvature-dependent aggregation of negatively charged lipids near the surface of the BAR domain. In the discussion, we suggest novel experiments using patch clamp techniques to analyze the binding dynamics of BAR proteins, as well as the possible role of BAR proteins in the fusion pore stability of exovesicles.

Abbreviations

BAR:

Bin/Amphiphysin/Rvs

IRSp53:

insulin receptor tyrosine kinase substrate p53

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Authors and Affiliations

  1. Laboratory of Physics, Faculty of Electrical Engineering, University of Ljubljana, Tržaška 25, SI-1000, Ljubljana, Slovenia

    Doron Kabaso & Aleš Iglič

  2. Institute of General Electrical Engineering, University of Rostock, Albert-Einstein-Straße 2, 18051, Rostock, Germany

    Ekaterina Gongadze & Ursula Van Rienen

  3. Laboratory of Neuroendocrinology and Molecular Cell Physiology, University of Ljubljana, Zaloška 4, Ljubljana, Slovenia

    Jernej Jorgačevski, Marko Kreft & Robert Zorec

  4. Celica Biomedical Center, Tehnoloski park 24, Ljubljana, Slovenia

    Jernej Jorgačevski, Marko Kreft & Robert Zorec

Authors
  1. Doron Kabaso
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  2. Ekaterina Gongadze
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  3. Jernej Jorgačevski
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  4. Marko Kreft
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  5. Ursula Van Rienen
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  6. Robert Zorec
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  7. Aleš Iglič
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Correspondence to Doron Kabaso.

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Kabaso, D., Gongadze, E., Jorgačevski, J. et al. Exploring the binding dynamics of BAR proteins. Cell Mol Biol Lett 16, 398–411 (2011). https://doi.org/10.2478/s11658-011-0013-0

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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