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Quantitative and dynamic expression profile of premature and active forms of the regional ADAM proteins during chicken brain development

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Abstract

The ADAM (A Disintegrin and Metalloprotease) family of transmembrane proteins plays important roles in embryogenesis and tissue formation based on their multiple functional domains. In the present study, for the first time, the expression patterns of the premature and the active forms of six members of the ADAM proteins — ADAM9, ADAM10, ADAM12, ADAM17, ADAM22 and ADAM23 — in distinct parts of the developing chicken brain were investigated by quantitative Western blot analysis from embryonic incubation day (E) 10 to E20. The results show that the premature and the active forms of various ADAM proteins are spatiotemporally regulated in different parts of the brain during development, suggesting that the ADAMs play a very important role during embryonic development.

Abbreviations

ADAM:

A Disintegrin and Metalloprotease

APP:

amyloid precursor protein

BCA:

bicinchoninic acid

CNS:

central nervous system

ECM:

extracellular matrix

EGF:

epidermal growth factor

EGFR:

epidermal growth factor receptor

ELISA:

enzyme-linked immunosorbent assay

HE-EGF:

heparin-binding EGFlike growth factor

kDa:

kilodalton

GAPDH:

glyceraldehyde-3-phosphate dehydrogenase

LGI1:

leucine-rich glioma inactivated 1

RT-PCR:

reverse transcriptionpolymerase chain reaction

SDS:

sodium dodecyl sulfate

TACE:

tumor necrosis factor alpha converting enzyme

TBST:

tris-buffered saline-Tween

TGF-α:

transforming growth factor-α

TNF-α:

tumor necrosis factor-α

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Correspondence to Jiankai Luo.

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Key words

  • ADAM
  • Gene expression
  • Protein
  • Brain development
  • Chicken