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Functional characterization of human Kindlin-2 core promoter identifies a key role of SP1 in Kindlin-2 transcriptional regulation

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Abstract

Kindlin-2 is a recently identified FERM and PH domain containing integrin interacting protein. Kindlin-2 is ubiquitously expressed in normal tissues. So far, much effort has been spent exploring the functional aspects of Kindlin-2. However, the transcriptional regulation of Kindlin-2 has not yet been investigated. In this study we identified and functionally characterized the promoter of the human Kindlin-2 gene. We show that the core promoter of Kindlin-2 is a 39 base pair long GC rich fragment located −122/-83 upstream of the Kindlin-2 transcription start site. Functional characterization of this core promoter region by both in silico as well as in vitro/in vivo analysis shows that the transcription factor SP1 plays an important role in regulation of Kindlin-2 expression.

Abbreviations

DBTSS:

database for transcription start site

FERM:

4.1 protein, ezrin, radixin and moesin

Mig-2:

mitogen-induced gene-2

PH:

pleckstrin homology

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Correspondence to Hongquan Zhang.

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Key words

  • Kindlin-2
  • FERMT2
  • PLEKHC1
  • Mig-2
  • Transcription factor SP1
  • Promoter
  • Transcription start site
  • Gene regulation
  • Cis-acting elements
  • CpG island
  • Cell migration
  • Integrin
  • Gene expression