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Differential fucosyltransferase IV expression in squamous carcinoma cells is regulated by promoter methylation

Cellular & Molecular Biology Letters volume 17, pages 206–216 (2012)Cite this article

Abstract

Enhanced fucosyltransferase IV (FUT4) expression correlates with increased tumor malignancy in many carcinomas. However, little is known about the regulation of FUT4 expression, and whether FUT4 expression is influenced by the methylation status of the FUT4 promoter is unclear. In this study, we demonstrated that FUT4 expression is negatively correlated with the methylation degree of a CpG island in the FUT4 promoter, suggesting that the methylation status of FUT4 promoter regulates the expression of FUT4. The results indicate that manipulating the methylation status of the FUT4 promoter to regulate FUT4 expression may be a novel approach in the treatment of malignant tumors.

Abbreviations

BSP:

bisulfite sequencing PCR

FUT4:

fucosyltransferase IV

MSP:

methylation-specific PCR

SCC:

squamous carcinoma cell

5-aza-dC:

5-aza-2-deoxycytidine

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian, Liaoning, People’s Republic of China

    Hongyan Li, Shaoming Tong, Li Han, Xuesong Yang & Qiu Yan

  2. College of Life Science, Liaoning Normal University, Dalian, Liaoning, People’s Republic of China

    Hongyan Li, Shaoming Tong & Hesheng Hou

  3. Department of Oncology, 1st Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, People’s Republic of China

    Jiwei Liu

  4. Departments of Dermatology and Pediatrics, Northwestern University’s Feinberg School of Medicine, Chicago, Illinois, USA

    Xiao-Qi Wang

Authors
  1. Hongyan Li
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  2. Shaoming Tong
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  3. Jiwei Liu
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  4. Li Han
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  5. Xuesong Yang
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  6. Hesheng Hou
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  7. Qiu Yan
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  8. Xiao-Qi Wang
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Correspondence to Xiao-Qi Wang.

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Li, H., Tong, S., Liu, J. et al. Differential fucosyltransferase IV expression in squamous carcinoma cells is regulated by promoter methylation. Cell Mol Biol Lett 17, 206–216 (2012). https://doi.org/10.2478/s11658-012-0003-x

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  • DOI: https://doi.org/10.2478/s11658-012-0003-x

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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