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Cellular & Molecular Biology Letters

Open Access

Autophagy favors Brucella melitensis survival in infected macrophages

  • Fei Guo1,
  • Hui Zhang2,
  • Chuangfu Chen1Email author,
  • Shengwei Hu2,
  • Yuanzhi Wang3,
  • Jun Qiao1,
  • Yan Ren3,
  • Ke Zhang1,
  • Yong Wang1 and
  • Guoqing Du1
Cellular & Molecular Biology LettersAn International Journal201217:9

Received: 20 July 2011

Accepted: 16 February 2012

Published: 24 February 2012


This study investigated the role of autophagy in the survival of the invasive bacterium Brucella melitensis strain 16M in murine macrophages. Here, Brucella melitensis 16M was found to trigger autophagosome formation, enhance autophagy flux and increase the expression level of the autophagy marker protein LC3-II. When autophagy was pharmacologically inhibited by 3-methyladenine (3-MA), Brucella replication efficiency was significantly decreased (p < 0.05). These results suggest that autophagy favors Brucella melitensis 16M survival in murine macrophages.

Key words

Autophagy Brucella melitensis 16MLC3-IILC3-IAutophagosomeAutophagy flux3-MethyladenineIntracellular pathogensMurine macrophage RAW264.7Autophagic vesicle