Open Access

Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.)

  • Ewa Olchowik1,
  • Karol Lotkowski1,
  • Saidmukhtar Mavlyanov2,
  • Nodira Abdullajanova2,
  • Maksim Ionov3,
  • Maria Bryszewska3 and
  • Maria Zamaraeva1Email author
Cellular & Molecular Biology LettersAn International Journal201217:14

https://doi.org/10.2478/s11658-012-0014-7

Received: 13 September 2011

Accepted: 29 March 2012

Published: 10 April 2012

Abstract

Erythrocytes are constantly exposed to ROS due to their function in the organism. High tension of oxygen, presence of hemoglobin iron and high concentration of polyunsaturated fatty acids in membrane make erythrocytes especially susceptible to oxidative stress. A comparison of the antioxidant activities of polyphenol-rich plant extracts containing hydrolysable tannins from sumac leaves (Rhus typhina L.) and condensed tannins from grape seeds (Vitis vinifera L.) showed that at the 5–50 μg/ml concentration range they reduced to the same extent hemolysis and glutathione, lipid and hemoglobin oxidation induced by erythrocyte treatment with 400 μM ONOO or 1 mM HClO. However, extract (condensed tannins) from grape seeds in comparison with extract (hydrolysable tannins) from sumac leaves stabilized erythrocytes in hypotonic NaCl solutions weakly. Our data indicate that both hydrolysable and condensed tannins significantly decrease the fluidity of the surface of erythrocyte membranes but the effect of hydrolysable ones was more profound. In conclusion, our results indicate that extracts from sumac leaves (hydrolysable tannins) and grape seeds (condensed tannins) are very effective protectors against oxidative damage in erythrocytes.

Key words

TanninsErythrocytesOxidative stressPeroxynitriteHypochlorous acidHemolysisFluorescence anisotropy

Notes

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