Open Access

Human adipose-derived stem cells for the treatment of intracerebral hemorrhage in rats via femoral intravenous injection

  • Kuo-Liang Yang1, 2Email author,
  • Jiunn-Tat Lee3,
  • Cheng-Yoong Pang4, 5,
  • Ting-Yi Lee1,
  • Shee-Ping Chen1,
  • Hock-Kean Liew4,
  • Shin-Yuan Chen6,
  • Tzu-Yung Chen7 and
  • Py-Yu Lin1
Cellular & Molecular Biology LettersAn International Journal201217:16

https://doi.org/10.2478/s11658-012-0016-5

Received: 27 September 2011

Accepted: 18 April 2012

Published: 28 April 2012

Abstract

Human adipose-derived stem cells (huADSC) were generated from fat tissue of a 65-year-old male donor. Flow cytometry and reverse transcription polymerase chain reaction (RT-PCR) analyses indicated that the huADSC express neural cell proteins (MAP2, GFAP, nestin and β-III tubulin), neurotrophic growth factors (BDNF and GDNF), and the chemotactic factor CXCR4 and its corresponding ligand CXCL12. In addition, huADSC expressed the characteristic mesenchymal stem cell (MSC) markers CD29, CD44, CD73, CD90, CD105 and HLA class I. The huADSC were employed, via a right femoral vein injection, to treat rats inflicted with experimental intracerebral hemorrhage (ICH). Behavioral measurement on the experimental animals, seven days after the huADSC therapy, showed a significant functional improvement in the rats with stem cell therapy in comparison with rats of the control group without the stem cell therapy. The injected huADSC were detectable in the brains of the huADSC treated rats as determined by histochemistry analysis, suggesting a role of the infused huADSC in facilitating functional recovery of the experimental animals with ICH induced stroke.

Key words

Adipose-derived stem cells Adult stem cells Adipose tissue Intracerebral hemorrhage Intravenous stem cell injection Neural stem cells Regenerative medicine Stem cell therapy Stroke Stromal cells

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