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Human adipose-derived stem cells for the treatment of intracerebral hemorrhage in rats via femoral intravenous injection

Cellular & Molecular Biology Letters volume 17, pages 376–392 (2012)Cite this article

Abstract

Human adipose-derived stem cells (huADSC) were generated from fat tissue of a 65-year-old male donor. Flow cytometry and reverse transcription polymerase chain reaction (RT-PCR) analyses indicated that the huADSC express neural cell proteins (MAP2, GFAP, nestin and β-III tubulin), neurotrophic growth factors (BDNF and GDNF), and the chemotactic factor CXCR4 and its corresponding ligand CXCL12. In addition, huADSC expressed the characteristic mesenchymal stem cell (MSC) markers CD29, CD44, CD73, CD90, CD105 and HLA class I. The huADSC were employed, via a right femoral vein injection, to treat rats inflicted with experimental intracerebral hemorrhage (ICH). Behavioral measurement on the experimental animals, seven days after the huADSC therapy, showed a significant functional improvement in the rats with stem cell therapy in comparison with rats of the control group without the stem cell therapy. The injected huADSC were detectable in the brains of the huADSC treated rats as determined by histochemistry analysis, suggesting a role of the infused huADSC in facilitating functional recovery of the experimental animals with ICH induced stroke.

Abbreviations

ADAS:

adipose-derived adult stem

ADSC:

adipose-derived stem cell

FCS:

fetal calf serum

hAdMSC:

adipose derived mesenchymal stem cell

hASC:

adipose tissue-derived stem cell

hATSC:

adipose tissue stromal cell

ICH:

intracerebral hemorrhage

MCAO:

middle cerebral artery occlusion

MSC:

mesenchymal stem cell

SVF:

stromal-vascular fraction

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Authors and Affiliations

  1. Buddhist Tzu Chi Stem Cells Center, Buddhist Tzu Chi Medical Center, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan

    Kuo-Liang Yang, Ting-Yi Lee, Shee-Ping Chen & Py-Yu Lin

  2. Institute of Medical Biotechnology, Buddhist Tzu Chi University, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan

    Kuo-Liang Yang

  3. Division of Plastic Surgery, Department of Surgery, Buddhist Tzu Chi Medical Center and Buddhist Tzu Chi University, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan

    Jiunn-Tat Lee

  4. Department of Research, Buddhist Tzu Chi Medical Center, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan

    Cheng-Yoong Pang & Hock-Kean Liew

  5. Graduate Institute of Clinical Medicine, Buddhist Tzu Chi University, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan

    Cheng-Yoong Pang

  6. Division of Functional Neuroscience, Department of Neurosurgery, Neuro-Medical Scientific Center, Buddhist Tzu Chi Medical Center, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan

    Shin-Yuan Chen

  7. Department of Neurosurgery, Buddhist Tzu Chi Medical Center Taichung Branch, Buddhist Compassion Relief Tzu Chi Foundation, Taichung, Taiwan

    Tzu-Yung Chen

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  1. Kuo-Liang Yang
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Correspondence to Kuo-Liang Yang.

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Yang, KL., Lee, JT., Pang, CY. et al. Human adipose-derived stem cells for the treatment of intracerebral hemorrhage in rats via femoral intravenous injection. Cell Mol Biol Lett 17, 376–392 (2012). https://doi.org/10.2478/s11658-012-0016-5

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  • DOI: https://doi.org/10.2478/s11658-012-0016-5

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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