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De novo synthesis of protein phosphatase 1A, magnesium dependent, alpha isoform (PPM1A) during oocyte maturation

Cellular & Molecular Biology Letters volume 17, pages 433–445 (2012)Cite this article

Abstract

Oocyte maturation in mammals is a multiple-stage process that generates fertilizable oocytes. Ovarian oocytes are arrested at prophase of the first meiotic division characterized by the presence of a germinal vesicle. Towards ovulation, the oocytes resume meiosis and proceed to the second metaphase in a process known as maturation; they undergo nuclear and cytoplasmic changes that are accompanied by translation and degradation of mRNA. Protein phosphatase 1A, magnesium dependent, alpha isoform (PPM1A), which belongs to the metal-dependent serine/threonine protein phosphatase family, is highly conserved during evolution. PPM1A plays a significant role in many cellular functions such as cell cycle progression, apoptosis and cellular differentiation. It works through diverse signaling pathways, including p38 MAP kinase JNK and transforming growth factor beta (TGF-β). Herein we report that PPM1A is expressed in mouse oocytes and that its mRNA level rises during oocyte maturation. Using quantitative real-time polymerase chain reaction (qPCR) and western blot analysis, we found that PPM1A mRNA is synthesized at the beginning of the maturation process and remains elevated in the mature oocytes, promoting the accumulation of PPM1A protein. Since PPM1A function is mainly affected by its level, we propose that it might have an important role in oocyte maturation.

Abbreviations

GV:

germinal vesicle

GVBD:

GV breakdown

hCG:

human chorionic gonadotropin

IVM:

in vitro maturation

LH:

luteinizing hormone

MII:

second meiotic division

MAPK:

mitogen-activated protein kinase

MPF:

maturation promoting factor

Phos-p38:

phospho-p38-MAPK

PP1:

protein phosphatase 1

PPM1A:

protein phosphates 1A, magnesium dependent, alpha isoform

RPL34:

ribosomal protein L34

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Authors and Affiliations

  1. Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv, Israel

    Dana Chuderland, Ruth Kaplan-Kraicer & Ruth Shalgi

  2. Cell Research and Immunology, The George S. Wise Faculty of Life Sciences, Tel-Aviv University, Ramat-Aviv, Tel-Aviv, 69978, Israel

    Zeev Dvashi, Daniella Ben-Meir & Sara Lavi

Authors
  1. Dana Chuderland
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  2. Zeev Dvashi
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  3. Ruth Kaplan-Kraicer
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  4. Daniella Ben-Meir
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  5. Ruth Shalgi
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  6. Sara Lavi
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Correspondence to Ruth Shalgi.

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Both authors contributed equally to the work

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Chuderland, D., Dvashi, Z., Kaplan-Kraicer, R. et al. De novo synthesis of protein phosphatase 1A, magnesium dependent, alpha isoform (PPM1A) during oocyte maturation. Cell Mol Biol Lett 17, 433–445 (2012). https://doi.org/10.2478/s11658-012-0022-7

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  • DOI: https://doi.org/10.2478/s11658-012-0022-7

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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